TY - JOUR
T1 - Drosophila mitochondrial transcription factor A
T2 - Characterization of its cDNA and expression pattern during development
AU - Takata, Kei ichi
AU - Yoshida, Hideki
AU - Hirose, Fumiko
AU - Yamaguchi, Masamitsu
AU - Kai, Mihoko
AU - Oshige, Masahiko
AU - Sakimoto, Ippei
AU - Koiwai, Osamu
AU - Sakaguchi, Kengo
PY - 2001/9/21
Y1 - 2001/9/21
N2 - We cloned a cDNA for Drosophila mitochondrial transcription factor A (D-mtTFA) and characterized the recombinant protein. In Drosophila Kc cells, D-mtTFA was localized in the mitochondria, but not in the nucleus. By repetitive precipitation with His-tag and PCR amplification, the consensus nucleotide sequence for D-mtTFA-binding was determined to be 5′-TTATC/G. The binding sequence was found to be clustered in the A+T region of mitochondrial DNA which is suggested to be a replication origin and promoter region for light strand and heavy strand. We found a DNA replication-related element (DRE)-like sequence located upstream of the transcription initiation site of the D-mtTFA gene and obtained results indicating that DRE-binding factor (DREF) can bind to the DRE-like sequence of the D-mtTFA gene. The data suggest that transcription of the D-mtTFA gene is under control of the DRE/DREF regulatory system. Based on these results, the functions of D-mtTFA were discussed in relation to mitochondrial biogenesis of Drosophila melanogaster.
AB - We cloned a cDNA for Drosophila mitochondrial transcription factor A (D-mtTFA) and characterized the recombinant protein. In Drosophila Kc cells, D-mtTFA was localized in the mitochondria, but not in the nucleus. By repetitive precipitation with His-tag and PCR amplification, the consensus nucleotide sequence for D-mtTFA-binding was determined to be 5′-TTATC/G. The binding sequence was found to be clustered in the A+T region of mitochondrial DNA which is suggested to be a replication origin and promoter region for light strand and heavy strand. We found a DNA replication-related element (DRE)-like sequence located upstream of the transcription initiation site of the D-mtTFA gene and obtained results indicating that DRE-binding factor (DREF) can bind to the DRE-like sequence of the D-mtTFA gene. The data suggest that transcription of the D-mtTFA gene is under control of the DRE/DREF regulatory system. Based on these results, the functions of D-mtTFA were discussed in relation to mitochondrial biogenesis of Drosophila melanogaster.
KW - DRE/DREF
KW - Drosophila melanogaster
KW - HMG box
KW - Mitochondria
KW - Transcription factor A
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U2 - 10.1006/bbrc.2001.5528
DO - 10.1006/bbrc.2001.5528
M3 - Article
C2 - 11554753
AN - SCOPUS:0035929340
SN - 0006-291X
VL - 287
SP - 474
EP - 483
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -