Drosophila mitochondrial transcription factor A: Characterization of its cDNA and expression pattern during development

Kei ichi Takata, Hideki Yoshida, Fumiko Hirose, Masamitsu Yamaguchi, Mihoko Kai, Masahiko Oshige, Ippei Sakimoto, Osamu Koiwai, Kengo Sakaguchi

Research output: Contribution to journalArticlepeer-review

Abstract

We cloned a cDNA for Drosophila mitochondrial transcription factor A (D-mtTFA) and characterized the recombinant protein. In Drosophila Kc cells, D-mtTFA was localized in the mitochondria, but not in the nucleus. By repetitive precipitation with His-tag and PCR amplification, the consensus nucleotide sequence for D-mtTFA-binding was determined to be 5′-TTATC/G. The binding sequence was found to be clustered in the A+T region of mitochondrial DNA which is suggested to be a replication origin and promoter region for light strand and heavy strand. We found a DNA replication-related element (DRE)-like sequence located upstream of the transcription initiation site of the D-mtTFA gene and obtained results indicating that DRE-binding factor (DREF) can bind to the DRE-like sequence of the D-mtTFA gene. The data suggest that transcription of the D-mtTFA gene is under control of the DRE/DREF regulatory system. Based on these results, the functions of D-mtTFA were discussed in relation to mitochondrial biogenesis of Drosophila melanogaster.

Original languageEnglish (US)
Pages (from-to)474-483
Number of pages10
JournalBiochemical and Biophysical Research Communications
Volume287
Issue number2
DOIs
StatePublished - Sep 21 2001
Externally publishedYes

Keywords

  • DRE/DREF
  • Drosophila melanogaster
  • HMG box
  • Mitochondria
  • Transcription factor A

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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