Driving midgut-specific expression and secretion of a foreign protein in transgenic mosquitoes with AgAper1 regulatory elements

E. G. Abraham, M. Donnelly-Doman, H. Fujioka, A. Ghosh, L. Moreira, Marcelo Jacobs-Lorena

Research output: Contribution to journalArticle

Abstract

The Anopheles gambiae adult peritrophic matrix protein 1 (AgAper1) regulatory elements were used to drive the expression of phospholipase A2 (PLA2), a protein known to disrupt malaria parasite development in mosquitoes. These AgAper1 regulatory elements were sufficient to promote the accumulation of PLA2 in midgut epithelial cells before a blood meal and its release into the lumen upon blood ingestion. Plasmodium berghei oocyst formation was reduced by ∼80% (74-91% range) in transgenic mosquitoes. Blood-seeking behaviour and survival of AgAper1-PLA2 transgenic mosquitoes were comparable to sibling wild-type mosquitoes, while fertility was substantially lower. Ultrastructural studies suggest that decreased fitness is a consequence of internal damage to midgut epithelial cells.

Original languageEnglish (US)
Pages (from-to)271-279
Number of pages9
JournalInsect Molecular Biology
Volume14
Issue number3
DOIs
StatePublished - Jun 2005

Fingerprint

Anopheles gambiae
peritrophic membrane
Culicidae
midgut
phospholipase A2
Phospholipases A2
secretion
genetically modified organisms
Blood
Proteins
epithelial cells
proteins
Epithelial Cells
Plasmodium berghei
Oocysts
blood
blood meal
oocysts
malaria
Malaria

Keywords

  • Peritrophic matrix protein 1
  • Phospholipase A2
  • Plasmodium
  • Transgenic mosquito
  • Transmission blocking

ASJC Scopus subject areas

  • Insect Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

Cite this

Driving midgut-specific expression and secretion of a foreign protein in transgenic mosquitoes with AgAper1 regulatory elements. / Abraham, E. G.; Donnelly-Doman, M.; Fujioka, H.; Ghosh, A.; Moreira, L.; Jacobs-Lorena, Marcelo.

In: Insect Molecular Biology, Vol. 14, No. 3, 06.2005, p. 271-279.

Research output: Contribution to journalArticle

Abraham, E. G. ; Donnelly-Doman, M. ; Fujioka, H. ; Ghosh, A. ; Moreira, L. ; Jacobs-Lorena, Marcelo. / Driving midgut-specific expression and secretion of a foreign protein in transgenic mosquitoes with AgAper1 regulatory elements. In: Insect Molecular Biology. 2005 ; Vol. 14, No. 3. pp. 271-279.
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