Driving midgut-specific expression and secretion of a foreign protein in transgenic mosquitoes with AgAper1 regulatory elements

E. G. Abraham, M. Donnelly-Doman, H. Fujioka, A. Ghosh, L. Moreira, M. Jacobs-Lorena

Research output: Contribution to journalArticle

Abstract

The Anopheles gambiae adult peritrophic matrix protein 1 (AgAper1) regulatory elements were used to drive the expression of phospholipase A2 (PLA2), a protein known to disrupt malaria parasite development in mosquitoes. These AgAper1 regulatory elements were sufficient to promote the accumulation of PLA2 in midgut epithelial cells before a blood meal and its release into the lumen upon blood ingestion. Plasmodium berghei oocyst formation was reduced by ∼80% (74-91% range) in transgenic mosquitoes. Blood-seeking behaviour and survival of AgAper1-PLA2 transgenic mosquitoes were comparable to sibling wild-type mosquitoes, while fertility was substantially lower. Ultrastructural studies suggest that decreased fitness is a consequence of internal damage to midgut epithelial cells.

Original languageEnglish (US)
Pages (from-to)271-279
Number of pages9
JournalInsect molecular biology
Volume14
Issue number3
DOIs
StatePublished - Jun 1 2005

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Keywords

  • Peritrophic matrix protein 1
  • Phospholipase A2
  • Plasmodium
  • Transgenic mosquito
  • Transmission blocking

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Insect Science

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