Dracorhodin perchlorate induced human breast cancer MCF-7 apoptosis through mitochondrial pathways.

Jing hua Yu, Gui bin Zheng, Chun yu Liu, Li ying Zhang, Hong mei Gao, Ya hong Zhang, Chun yan Dai, Lin Huang, Xian ying Meng, Wen Yan Zhang, Xiao fang Yu

Research output: Contribution to journalArticle

Abstract

Dracorhodin perchlorate (DP) was a synthetic analogue of the antimicrobial anthocyanin red pigment dracorhodin. It was reported that DP could induce apoptosis in human prostate cancer, human gastric tumor cells and human melanoma, but the cytotoxic effect of DP on human breast cancer was not investigated. This study would investigate whether DP was a candidate chemical of anti-human breast cancer. The MTT assay reflected the number of viable cells through measuring the activity of cellular enzymes. Phase contrast microscopy visualized cell morphology. Fluorescence microscopy detected nuclear fragmentation after Hoechst 33258 staining. Flowcytometric analysis of Annexin V-PI staining and Rodamine 123 staining was used to detect cell apoptosis and mitochondrial membrane potential (MMP). Real time PCR detected mRNA level. Western blot examined protein expression. DP dose and time-dependently inhibited the growth of MCF-7 cells. DP inhibited MCF-7 cell growth through apoptosis. DP regulated the expression of Bcl-2 and Bax, which were mitochondrial pathway proteins, to decrease MMP, and DP promoted the transcription of Bax and inhibited Bcl-2. Apoptosis-inducing factor (AIF) and cytochrome c which localized in mitochondrial in physiological condition were released into cytoplasm when MMP was decreased. DP activated caspase-9, which was the downstream of mitochondrial pathway. Therefore DP decreased MMP to release AIF and cytochrome c into cytoplasm, further activating caspase 9, lastly led to apoptosis. Therefore DP was a candidate for anti-breast cancer, DP induced apoptosis of MCF-7 through mitochondrial pathway.

Original languageEnglish (US)
Pages (from-to)1149-1156
Number of pages8
JournalInternational Journal of Medical Sciences
Volume10
Issue number9
StatePublished - 2013
Externally publishedYes

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Apoptosis
Breast Neoplasms
Mitochondrial Membrane Potential
Apoptosis Inducing Factor
Caspase 9
MCF-7 Cells
Staining and Labeling
Cytochromes c
dracorhodin
Cytoplasm
Bisbenzimidazole
Phase-Contrast Microscopy
Anthocyanins
Annexin A5
Mitochondrial Proteins
Growth
Fluorescence Microscopy
Stomach Neoplasms
Real-Time Polymerase Chain Reaction
Melanoma

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Yu, J. H., Zheng, G. B., Liu, C. Y., Zhang, L. Y., Gao, H. M., Zhang, Y. H., ... Yu, X. F. (2013). Dracorhodin perchlorate induced human breast cancer MCF-7 apoptosis through mitochondrial pathways. International Journal of Medical Sciences, 10(9), 1149-1156.

Dracorhodin perchlorate induced human breast cancer MCF-7 apoptosis through mitochondrial pathways. / Yu, Jing hua; Zheng, Gui bin; Liu, Chun yu; Zhang, Li ying; Gao, Hong mei; Zhang, Ya hong; Dai, Chun yan; Huang, Lin; Meng, Xian ying; Zhang, Wen Yan; Yu, Xiao fang.

In: International Journal of Medical Sciences, Vol. 10, No. 9, 2013, p. 1149-1156.

Research output: Contribution to journalArticle

Yu, JH, Zheng, GB, Liu, CY, Zhang, LY, Gao, HM, Zhang, YH, Dai, CY, Huang, L, Meng, XY, Zhang, WY & Yu, XF 2013, 'Dracorhodin perchlorate induced human breast cancer MCF-7 apoptosis through mitochondrial pathways.', International Journal of Medical Sciences, vol. 10, no. 9, pp. 1149-1156.
Yu, Jing hua ; Zheng, Gui bin ; Liu, Chun yu ; Zhang, Li ying ; Gao, Hong mei ; Zhang, Ya hong ; Dai, Chun yan ; Huang, Lin ; Meng, Xian ying ; Zhang, Wen Yan ; Yu, Xiao fang. / Dracorhodin perchlorate induced human breast cancer MCF-7 apoptosis through mitochondrial pathways. In: International Journal of Medical Sciences. 2013 ; Vol. 10, No. 9. pp. 1149-1156.
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abstract = "Dracorhodin perchlorate (DP) was a synthetic analogue of the antimicrobial anthocyanin red pigment dracorhodin. It was reported that DP could induce apoptosis in human prostate cancer, human gastric tumor cells and human melanoma, but the cytotoxic effect of DP on human breast cancer was not investigated. This study would investigate whether DP was a candidate chemical of anti-human breast cancer. The MTT assay reflected the number of viable cells through measuring the activity of cellular enzymes. Phase contrast microscopy visualized cell morphology. Fluorescence microscopy detected nuclear fragmentation after Hoechst 33258 staining. Flowcytometric analysis of Annexin V-PI staining and Rodamine 123 staining was used to detect cell apoptosis and mitochondrial membrane potential (MMP). Real time PCR detected mRNA level. Western blot examined protein expression. DP dose and time-dependently inhibited the growth of MCF-7 cells. DP inhibited MCF-7 cell growth through apoptosis. DP regulated the expression of Bcl-2 and Bax, which were mitochondrial pathway proteins, to decrease MMP, and DP promoted the transcription of Bax and inhibited Bcl-2. Apoptosis-inducing factor (AIF) and cytochrome c which localized in mitochondrial in physiological condition were released into cytoplasm when MMP was decreased. DP activated caspase-9, which was the downstream of mitochondrial pathway. Therefore DP decreased MMP to release AIF and cytochrome c into cytoplasm, further activating caspase 9, lastly led to apoptosis. Therefore DP was a candidate for anti-breast cancer, DP induced apoptosis of MCF-7 through mitochondrial pathway.",
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AU - Dai, Chun yan

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