DRβ1*1501, DQβ1*0602 donor allelic haplotype and humoral antibody episodes of acute renal allograft rejection

Morgan Cowan, Venkat K.R. Mannam, Taryn Green, Joshua R. Goodin, Robert E. Lewis, Steven A. Bigler, Julius M. Cruse

Research output: Contribution to journalArticle

Abstract

The present investigation was designed to show the effect of molecular HLA class II DR and DQ allelic differences between donor and recipient on humoral antibody rejection identified by C4d peritubular capillary staining. The hypothesis is that expression of the DRβ1*1501, DQβ*0602 allele in the donor kidney increases the likelihood of humoral antibody rejection. We found that 67% (n = 18) of DR15 and/or DQ6 haplotype donor kidneys induced humoral antibody renal allograft rejection; 35% (n = 40) of DR15 and/or DQ6 haplotype donor kidneys failed to induce humoral antibody renal allograft rejection (p = 0.02). 42% (n = 31) of C4d+ recipients had donors with DR15; 17% (n = 42) of C4d recipients had donors with HLA-DR15 (p = 0.01).We compared donor haplotype alleles of 4 C4d+ with 6 C4d- recipients by high resolution molecular typing; 3 of 4 C4d+ recipients had a donor with the DRβ1*1501/DQβ1*0602 allele. This allele was absent in all C4d- donors. 35% of C4d+ recipients had 2 DR mismatches when compared to 36% of C4d- recipients. Our results, suggest that the DRβ1*1501, DQβ1*0602 allele in the donor kidney increases the risk of humoral antibody episodes of acute rejection, and signals the need for C4d staining of renal biopsies. Future analysis of additional donor and recipient haplotypes will establish whether or not this is a useful predictor of humoral rejection episodes.

Original languageEnglish (US)
Pages (from-to)135-139
Number of pages5
JournalExperimental and Molecular Pathology
Volume89
Issue number2
DOIs
StatePublished - Oct 1 2010

Keywords

  • C4d staining
  • Donor allele
  • Humoral rejection
  • Renal allograft

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Molecular Biology
  • Clinical Biochemistry

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    Cowan, M., Mannam, V. K. R., Green, T., Goodin, J. R., Lewis, R. E., Bigler, S. A., & Cruse, J. M. (2010). DRβ1*1501, DQβ1*0602 donor allelic haplotype and humoral antibody episodes of acute renal allograft rejection. Experimental and Molecular Pathology, 89(2), 135-139. https://doi.org/10.1016/j.yexmp.2010.06.008