Downregulation of cornulin in esophageal squamous cell carcinoma

Harsh Pawar, Jagadeesha Maharudraiah, Manoj Kumar Kashyap, Jyoti Sharma, Srinivas Manda Srikanth, Robin Choudhary, Sandip Chavan, Gajanan Sathe, Hosuru Chikkalingaiah Manju, Kariyanakatte Veeraiah Veerendra Kumar, Manavalan Vijayakumar, Ravi Sirdeshmukh, Hindahally Chandregowda Harsha, Thottethodi Subrahmanya Keshava Prasad, Akhilesh Pandey, Rekha Vijay Kumar

Research output: Contribution to journalArticle

Abstract

Early events in the development of esophageal squamous cell carcinoma (ESCC) are poorly understood and many of the key molecules involved have not yet been identified. We previously used isobaric tags for a relative and absolute quantitation (iTRAQ)-based quantitative proteomics approach to identify differentially expressed proteins in ESCC tissue as compared to the adjacent normal mucosa. Cornulin was identified as one of the major downregulated molecules in ESCC. Cornulin is a member of the S100 fused-type protein family, which has an EF-hand calcium binding motif and multiple tandem repeats of specific peptide motifs. Cornulin was 5-fold downregulated in ESCC as compared to normal epithelium mirroring our previous findings in a gene expression study of ESCC. In the present study, we performed immunohistochemical validation of cornulin (CRNN) in a larger set of patients with ESCC. Downregulation of cornulin was observed in 89% (n= 239) of 266 different ESCC tissues arrayed on tissue microarrays (TMAs). Expression of cornulin was observed in the prickle and functional cell layers of normal esophageal mucosa, localized predominantly in the cytoplasm and perinuclear region. The large majority of ESCC cases had little or no expression of cornulin in the carcinoma or stroma. These findings suggest that cornulin is an important molecule in normal esophageal pathology and is likely lost during the conversion of normal to neoplastic epithelium.

Original languageEnglish (US)
Pages (from-to)89-99
Number of pages11
JournalActa Histochemica
Volume115
Issue number2
DOIs
StatePublished - Mar 2013

Keywords

  • C1Orf10
  • Epidermal differentiation complex
  • Loss of heterozygosity
  • Mass spectrometry
  • S100 gene family
  • Squamous epithelial-induced stress protein of 53kDa
  • Stress response protein

ASJC Scopus subject areas

  • Cell Biology
  • Histology

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  • Cite this

    Pawar, H., Maharudraiah, J., Kashyap, M. K., Sharma, J., Srikanth, S. M., Choudhary, R., Chavan, S., Sathe, G., Manju, H. C., Kumar, K. V. V., Vijayakumar, M., Sirdeshmukh, R., Harsha, H. C., Prasad, T. S. K., Pandey, A., & Kumar, R. V. (2013). Downregulation of cornulin in esophageal squamous cell carcinoma. Acta Histochemica, 115(2), 89-99. https://doi.org/10.1016/j.acthis.2012.04.003