Down syndrome mouse models Ts65Dn, Ts1Cje, and Ms1Cje/Ts65Dn exhibit variable severity of cerebellar phenotypes

L. E. Olson, R. J. Roper, L. L. Baxter, E. J. Carlson, C. J. Epstein, Roger H. Reeves

Research output: Contribution to journalArticle

Abstract

Two mouse models are widely used for Down syndrome (DS) research. The Ts65Dn mouse carries a small chromosome derived primarily from mouse chromosome 16, causing dosage imbalance for approximately half of human chromosome 21 orthologs. These mice have cerebellar pathology with direct parallels to DS. The Ts1Cje mouse, containing a translocated chromosome 16, is at dosage imbalance for 67% of the genes triplicated in Ts65Dn. We quantified cerebellar volume and granule cell and Purkinje cell density in Ts1Cje. Cerebellar volume was significantly affected to the same degree in Ts1Cje and Ts65Dn, despite that Ts1Cje has fewer triplicated genes. However, dosage imbalance in Ts1Cje had little effect on granule cell and Purkinje cell density. Several mice with dosage imbalance for the segment of the Ts65Dn chromosome not triplicated in Ts1Cje had phenotypes that contrasted with those in Ts1Cje. These observations do not readily differentiate between two prevalent hypotheses for gene action in DS.

Original languageEnglish (US)
Pages (from-to)581-589
Number of pages9
JournalDevelopmental Dynamics
Volume230
Issue number3
DOIs
StatePublished - Jul 1 2004

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Keywords

  • Cell density
  • Cerebellum
  • Down syndrome
  • Mouse model
  • Trisomy
  • Volume

ASJC Scopus subject areas

  • Developmental Biology

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