Down-regulation of IL-8 expression in human airway epithelial cells through helper-dependent adenoviral-mediated RNA interference

Huibi Cao, Anan Wang, Bernard Martin, David R. Koehler, Pamela L. Zeitlin, A. Keith Tanawell, Jim Hu

Research output: Contribution to journalArticle

Abstract

Interleukin (IL)-8 is a potent neutrophil chemotactic factor and a crucial mediator in neutrophil-dependent inflammation. Various cell types produce IL-8, either in response to external stimuli such as cytokines or bacterial infection, or after malignant transformation. Anti-IL-8 strategies have been considered for anti-inflammatory therapy. In this paper we demonstrate that the RNA interference technique can be used to efficiently down-regulate IL-8 protein expression in airway epithelial cells. We used a helper-dependent adenoviral vector to express a small hairpin (sh)RNA targeting human IL-8 in cultured airway epithelial cells (IB3-1, Cftr-/-; C38, Cftr-corrected) stimulated with TNF-α, IL-1β or heat-inactivated Burkholderia cenocepacia. Stimulated IL-8 expression in IB3-1 and C38 cells was significantly reduced by shRNA expression. The shRNA targeting IL-8 had no effect on the activation of NF-κB, or on the protein levels of IκB or IL-6, suggesting that this anti-IL-8 strategy was highly specific, and therefore may offer potential for the treatment of inflammatory diseases.

Original languageEnglish (US)
Pages (from-to)111-119
Number of pages9
JournalCell Research
Volume15
Issue number2
DOIs
StatePublished - 2005

Fingerprint

RNA Interference
Interleukin-8
Down-Regulation
Epithelial Cells
Small Interfering RNA
Burkholderia cenocepacia
Interleukin-1
Bacterial Infections
Interleukin-6
Proteins
Neutrophils
Anti-Inflammatory Agents
Hot Temperature
Cytokines
Inflammation

Keywords

  • Chemokine
  • Cystic fibrosis
  • Helper-dependent adenoviral vector
  • Inflammation
  • Interleukin-8
  • Neutrophil
  • RNA interference

ASJC Scopus subject areas

  • Cell Biology

Cite this

Down-regulation of IL-8 expression in human airway epithelial cells through helper-dependent adenoviral-mediated RNA interference. / Cao, Huibi; Wang, Anan; Martin, Bernard; Koehler, David R.; Zeitlin, Pamela L.; Tanawell, A. Keith; Hu, Jim.

In: Cell Research, Vol. 15, No. 2, 2005, p. 111-119.

Research output: Contribution to journalArticle

Cao, Huibi ; Wang, Anan ; Martin, Bernard ; Koehler, David R. ; Zeitlin, Pamela L. ; Tanawell, A. Keith ; Hu, Jim. / Down-regulation of IL-8 expression in human airway epithelial cells through helper-dependent adenoviral-mediated RNA interference. In: Cell Research. 2005 ; Vol. 15, No. 2. pp. 111-119.
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