Abstract
Interleukin (IL)-8 is a potent neutrophil chemotactic factor and a crucial mediator in neutrophil-dependent inflammation. Various cell types produce IL-8, either in response to external stimuli such as cytokines or bacterial infection, or after malignant transformation. Anti-IL-8 strategies have been considered for anti-inflammatory therapy. In this paper we demonstrate that the RNA interference technique can be used to efficiently down-regulate IL-8 protein expression in airway epithelial cells. We used a helper-dependent adenoviral vector to express a small hairpin (sh)RNA targeting human IL-8 in cultured airway epithelial cells (IB3-1, Cftr-/-; C38, Cftr-corrected) stimulated with TNF-α, IL-1β or heat-inactivated Burkholderia cenocepacia. Stimulated IL-8 expression in IB3-1 and C38 cells was significantly reduced by shRNA expression. The shRNA targeting IL-8 had no effect on the activation of NF-κB, or on the protein levels of IκB or IL-6, suggesting that this anti-IL-8 strategy was highly specific, and therefore may offer potential for the treatment of inflammatory diseases.
Original language | English (US) |
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Pages (from-to) | 111-119 |
Number of pages | 9 |
Journal | Cell Research |
Volume | 15 |
Issue number | 2 |
DOIs | |
State | Published - 2005 |
Externally published | Yes |
Keywords
- Chemokine
- Cystic fibrosis
- Helper-dependent adenoviral vector
- Inflammation
- Interleukin-8
- Neutrophil
- RNA interference
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology