Double-strand breaks can initiate meiotic recombination in S. cerevisiae

Alex L. Kolodkin; Amar J.S. Klar; Franklin W. Stahl

doi:10.1016/0092-8674(86)90349-1

Double-strand breaks can initiate meiotic recombination in S. cerevisiae

Alex L. Kolodkin, Amar J.S. Klar, Franklin W. Stahl

Research output: Contribution to journal › Article › peer-review

55 Scopus citations

Abstract

We investigated the effects of double-strand breaks on meiotic recombination in yeast. A double-strand break was introduced at the MATa locus by sporulation of a MATα_inc MATa diploid under inducing conditions for the HO-encoded endonuclease; 14% of the resulting tetrads had undergone 4α:0a conversion. Conversion at MAT was associated with co-conversion of a closely linked marker and an increased recombination frequency for flanking markers. We also studied the sporulation products of a diploid heterozygous at the HIS4 locus for an insertion of a 100 bp fragment of MATa containing the HO endonuclease cut site. Under inducing conditions, a significant number of tetrads were formed that had undergone gene conversions in favor of the HIS4⁺ allele. Although double-strand breaks can initiate meiotic recombination in yeast, the data suggest that they do not normally do so.

Original language	English (US)
Pages (from-to)	733-740
Number of pages	8
Journal	Cell
Volume	46
Issue number	5
DOIs	https://doi.org/10.1016/0092-8674(86)90349-1
State	Published - Aug 29 1986
Externally published	Yes

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology

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10.1016/0092-8674(86)90349-1

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title = "Double-strand breaks can initiate meiotic recombination in S. cerevisiae",

abstract = "We investigated the effects of double-strand breaks on meiotic recombination in yeast. A double-strand break was introduced at the MATa locus by sporulation of a MATαinc MATa diploid under inducing conditions for the HO-encoded endonuclease; 14% of the resulting tetrads had undergone 4α:0a conversion. Conversion at MAT was associated with co-conversion of a closely linked marker and an increased recombination frequency for flanking markers. We also studied the sporulation products of a diploid heterozygous at the HIS4 locus for an insertion of a 100 bp fragment of MATa containing the HO endonuclease cut site. Under inducing conditions, a significant number of tetrads were formed that had undergone gene conversions in favor of the HIS4+ allele. Although double-strand breaks can initiate meiotic recombination in yeast, the data suggest that they do not normally do so.",

author = "Kolodkin, {Alex L.} and Klar, {Amar J.S.} and Stahl, {Franklin W.}",

note = "Funding Information: We are grateful to the following people for their various contributions to this work: Elizabeth Cooksey, Gerry Fink, Jim Haber, Jim Hicks, Robert Jensen, Hanna Klein, Ann Hagemann, Animesh Ray, Janet Rosenberg, Susan Rosenberg, Rodney Rothstein, lmran Siddiqi, George Sprague, Jeff Strathern. David Thaler, and Carl Yamashiro. A. L. K. is supported by National Institutes of Health predoctoral fellowship PHS GM07759. This work was supported by NIH grant GM 33677 and National Science Foundation PLM 8409843 to F. W. S. and by NIH grant GM 25687 to A. J. S. K. F. W. S. is an American Cancer Society Research Professor of Molecular Genetics. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.",

year = "1986",

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doi = "10.1016/0092-8674(86)90349-1",

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AU - Kolodkin, Alex L.

AU - Klar, Amar J.S.

AU - Stahl, Franklin W.

N1 - Funding Information: We are grateful to the following people for their various contributions to this work: Elizabeth Cooksey, Gerry Fink, Jim Haber, Jim Hicks, Robert Jensen, Hanna Klein, Ann Hagemann, Animesh Ray, Janet Rosenberg, Susan Rosenberg, Rodney Rothstein, lmran Siddiqi, George Sprague, Jeff Strathern. David Thaler, and Carl Yamashiro. A. L. K. is supported by National Institutes of Health predoctoral fellowship PHS GM07759. This work was supported by NIH grant GM 33677 and National Science Foundation PLM 8409843 to F. W. S. and by NIH grant GM 25687 to A. J. S. K. F. W. S. is an American Cancer Society Research Professor of Molecular Genetics. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.

PY - 1986/8/29

Y1 - 1986/8/29

N2 - We investigated the effects of double-strand breaks on meiotic recombination in yeast. A double-strand break was introduced at the MATa locus by sporulation of a MATαinc MATa diploid under inducing conditions for the HO-encoded endonuclease; 14% of the resulting tetrads had undergone 4α:0a conversion. Conversion at MAT was associated with co-conversion of a closely linked marker and an increased recombination frequency for flanking markers. We also studied the sporulation products of a diploid heterozygous at the HIS4 locus for an insertion of a 100 bp fragment of MATa containing the HO endonuclease cut site. Under inducing conditions, a significant number of tetrads were formed that had undergone gene conversions in favor of the HIS4+ allele. Although double-strand breaks can initiate meiotic recombination in yeast, the data suggest that they do not normally do so.

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Double-strand breaks can initiate meiotic recombination in S. cerevisiae

Abstract

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