Double-minute chromatin bodies in HL-60 leukemia cells sensitive and resistant to differentiation inducing agents

G. Schwartsmann, K. Madan, Y. Heins, H. M. Pinedo, A. Leyva

Research output: Contribution to journalArticlepeer-review

Abstract

We studied the chromosome characteristics of HL-60 promyelocytic leukemia cells sensitive and resistant to differentiation inducing agents (DI). The karyotypic analysis of sensitive (HL-60 S) and resistant (HL-60 R) cells revealed the presence of identical chromosome abnormalities such as loss of chromosomes 5, 9, 10, 14, 16, 17 and X; and gain of chromosome 18. HL-60 S and HL-60 R cells also share five common markers. The difference between the two cell lines consisted essentially of the loss of an unidentifiable chromosome segment in the HL-60 R cell line. In addition, the two sublines showed marked differences in the content of double-minute chromatin bodies (DM), which were abundant in HL-60 S but rarely found in HL-60 R cells. Contrary to a previous report by others, there was no evidence of chromosome rearrangement of the DM as homogeneously stained regions (HSR) or abnormally banding regions (ABR) in the resistant HL-60 R cells. The presence of DM as an expression of gene amplification may be of relevance in the determination of susceptibility of HL-60 cells to DI.

Original languageEnglish (US)
Pages (from-to)651-655
Number of pages5
JournalCell Biology International Reports
Volume11
Issue number9
DOIs
StatePublished - 1987
Externally publishedYes

ASJC Scopus subject areas

  • Cell Biology

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