Double-antibody immunoenzyme assay for human prostatic acid phosphatase

B. K. Choe, E. J. Pontes, M. K. Dong, N. R. Rose

Research output: Contribution to journalArticlepeer-review

Abstract

We compare the double-antibody radioimmunoassay (RIA) and immunoenzyme assay (IEA) for measuring the concentration of prostatic acid phosphatase in human serum. Experimental details and assay performance of the two methods are outlined. Mean values for 385 normal persons were 1.02 (SD 1.32) μg/L by IEA, 2.69 (SD 1.8) μg/L by RIA. Results of the two methods was highly correlated [r = 0.9813, y(RIA) = 0.35x(IEA) + 0.42, p<0.001]. If we choose x̄ + 2 SD as the normal range, 3-10% false positives were seen.

Original languageEnglish (US)
Pages (from-to)1854-1859
Number of pages6
JournalClinical chemistry
Volume26
Issue number13
DOIs
StatePublished - 1980

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, medical

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