Double-antibody immunoenzyme assay for human prostatic acid phosphatase

B. K. Choe; E. J. Pontes; M. K. Dong; N. R. Rose

doi:10.1093/clinchem/26.13.1854

Double-antibody immunoenzyme assay for human prostatic acid phosphatase

B. K. Choe, E. J. Pontes, M. K. Dong, N. R. Rose

School of Medicine

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

We compare the double-antibody radioimmunoassay (RIA) and immunoenzyme assay (IEA) for measuring the concentration of prostatic acid phosphatase in human serum. Experimental details and assay performance of the two methods are outlined. Mean values for 385 normal persons were 1.02 (SD 1.32) μg/L by IEA, 2.69 (SD 1.8) μg/L by RIA. Results of the two methods was highly correlated [r = 0.9813, y(RIA) = 0.35x(IEA) + 0.42, p<0.001]. If we choose x̄ + 2 SD as the normal range, 3-10% false positives were seen.

Original language	English (US)
Pages (from-to)	1854-1859
Number of pages	6
Journal	Clinical chemistry
Volume	26
Issue number	13
DOIs	https://doi.org/10.1093/clinchem/26.13.1854
State	Published - 1980

ASJC Scopus subject areas

Clinical Biochemistry
Biochemistry, medical

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abstract = "We compare the double-antibody radioimmunoassay (RIA) and immunoenzyme assay (IEA) for measuring the concentration of prostatic acid phosphatase in human serum. Experimental details and assay performance of the two methods are outlined. Mean values for 385 normal persons were 1.02 (SD 1.32) μg/L by IEA, 2.69 (SD 1.8) μg/L by RIA. Results of the two methods was highly correlated [r = 0.9813, y(RIA) = 0.35x(IEA) + 0.42, p<0.001]. If we choose {\=x} + 2 SD as the normal range, 3-10% false positives were seen.",

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AU - Choe, B. K.

AU - Pontes, E. J.

AU - Dong, M. K.

AU - Rose, N. R.

PY - 1980

Y1 - 1980

N2 - We compare the double-antibody radioimmunoassay (RIA) and immunoenzyme assay (IEA) for measuring the concentration of prostatic acid phosphatase in human serum. Experimental details and assay performance of the two methods are outlined. Mean values for 385 normal persons were 1.02 (SD 1.32) μg/L by IEA, 2.69 (SD 1.8) μg/L by RIA. Results of the two methods was highly correlated [r = 0.9813, y(RIA) = 0.35x(IEA) + 0.42, p<0.001]. If we choose x̄ + 2 SD as the normal range, 3-10% false positives were seen.

AB - We compare the double-antibody radioimmunoassay (RIA) and immunoenzyme assay (IEA) for measuring the concentration of prostatic acid phosphatase in human serum. Experimental details and assay performance of the two methods are outlined. Mean values for 385 normal persons were 1.02 (SD 1.32) μg/L by IEA, 2.69 (SD 1.8) μg/L by RIA. Results of the two methods was highly correlated [r = 0.9813, y(RIA) = 0.35x(IEA) + 0.42, p<0.001]. If we choose x̄ + 2 SD as the normal range, 3-10% false positives were seen.

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Double-antibody immunoenzyme assay for human prostatic acid phosphatase

Abstract

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