Dot-based ELISA and RIA: Two rapid assays that screen hybridoma supernatants against whole live cells

M. Stya, R. Wahl, W. H. Beierwaltes

Research output: Contribution to journalArticle

Abstract

Two assays are described that are suitable for the screening of large numbers of hybridoma supernatants as well as for the screening of a wide range of tissues for the distribution of a given cell surface antigen. These assays use whole live cells as targets, avoiding fixation or extraction, which could alter the antigenic structural profile. The assays are rapid, simple and inexpensive. Their advantages and applications are discussed.

Original languageEnglish (US)
Pages (from-to)75-81
Number of pages7
JournalJournal of Immunological Methods
Volume73
Issue number1
DOIs
StatePublished - Oct 12 1984

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Keywords

  • ELISA
  • hybridoma screening
  • radioimmunoassay

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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