Dopamine receptor binding: Specificity, localization and regulation by ions and guanyl nucleotides

Ian Creese; Timothy Prosser; Solomon H. Snyder

doi:10.1016/0024-3205(78)90160-1

Dopamine receptor binding: Specificity, localization and regulation by ions and guanyl nucleotides

Ian Creese, Timothy Prosser, Solomon H. Snyder

School of Medicine

Research output: Contribution to journal › Article › peer-review

93 Scopus citations

Abstract

³H-Spiroperidol labels dopamine receptors in rats striatum but in frontal cortex and hippocampus ³H-spiroperidol labels serotonin receptors. The agonists ³H-ADTN and ³H-apomorphine label rat striatal dopamine receptors. Comparison with calf striatal binding indicates a species difference in ³H-apomorphine binding. Drug displacement and lesion studies suggest that in the rat ³H-apomorphine labels two distinct dopamine receptors, one associated with the dopamine-sensitive adenylate cyclase and the other with presynaptic dopamine receptors also labeled by ³H-spiroperidol. Whereas divalent cations increase specific dopamine receptor binding of ³H-agonists and ³H-antagonists, ³H-agonist binding is selectively decreased by some guanyl nucleotides.

Original language	English (US)
Pages (from-to)	495-499
Number of pages	5
Journal	Life Sciences
Volume	23
Issue number	5
DOIs	https://doi.org/10.1016/0024-3205(78)90160-1
State	Published - Aug 7 1978

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)
Pharmacology, Toxicology and Pharmaceutics(all)

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10.1016/0024-3205(78)90160-1

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@article{9a9be053de4f43ec8e301896bd75cbf3,

title = "Dopamine receptor binding: Specificity, localization and regulation by ions and guanyl nucleotides",

abstract = "3H-Spiroperidol labels dopamine receptors in rats striatum but in frontal cortex and hippocampus 3H-spiroperidol labels serotonin receptors. The agonists 3H-ADTN and 3H-apomorphine label rat striatal dopamine receptors. Comparison with calf striatal binding indicates a species difference in 3H-apomorphine binding. Drug displacement and lesion studies suggest that in the rat 3H-apomorphine labels two distinct dopamine receptors, one associated with the dopamine-sensitive adenylate cyclase and the other with presynaptic dopamine receptors also labeled by 3H-spiroperidol. Whereas divalent cations increase specific dopamine receptor binding of 3H-agonists and 3H-antagonists, 3H-agonist binding is selectively decreased by some guanyl nucleotides.",

author = "Ian Creese and Timothy Prosser and Snyder, {Solomon H.}",

note = "Funding Information: Acknowledsements: Douglas Reynolds, Ted Usdin and Kim Stewart are thanked for their contributions. Supported by MH-18501 and grants to I.C. from Merck, Sharpe and Dohme and J.H.U.M.S. Manuscript prepared by Pamela Morgan. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.",

year = "1978",

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doi = "10.1016/0024-3205(78)90160-1",

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pages = "495--499",

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T2 - Specificity, localization and regulation by ions and guanyl nucleotides

AU - Creese, Ian

AU - Prosser, Timothy

AU - Snyder, Solomon H.

N1 - Funding Information: Acknowledsements: Douglas Reynolds, Ted Usdin and Kim Stewart are thanked for their contributions. Supported by MH-18501 and grants to I.C. from Merck, Sharpe and Dohme and J.H.U.M.S. Manuscript prepared by Pamela Morgan. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.

PY - 1978/8/7

Y1 - 1978/8/7

N2 - 3H-Spiroperidol labels dopamine receptors in rats striatum but in frontal cortex and hippocampus 3H-spiroperidol labels serotonin receptors. The agonists 3H-ADTN and 3H-apomorphine label rat striatal dopamine receptors. Comparison with calf striatal binding indicates a species difference in 3H-apomorphine binding. Drug displacement and lesion studies suggest that in the rat 3H-apomorphine labels two distinct dopamine receptors, one associated with the dopamine-sensitive adenylate cyclase and the other with presynaptic dopamine receptors also labeled by 3H-spiroperidol. Whereas divalent cations increase specific dopamine receptor binding of 3H-agonists and 3H-antagonists, 3H-agonist binding is selectively decreased by some guanyl nucleotides.

AB - 3H-Spiroperidol labels dopamine receptors in rats striatum but in frontal cortex and hippocampus 3H-spiroperidol labels serotonin receptors. The agonists 3H-ADTN and 3H-apomorphine label rat striatal dopamine receptors. Comparison with calf striatal binding indicates a species difference in 3H-apomorphine binding. Drug displacement and lesion studies suggest that in the rat 3H-apomorphine labels two distinct dopamine receptors, one associated with the dopamine-sensitive adenylate cyclase and the other with presynaptic dopamine receptors also labeled by 3H-spiroperidol. Whereas divalent cations increase specific dopamine receptor binding of 3H-agonists and 3H-antagonists, 3H-agonist binding is selectively decreased by some guanyl nucleotides.

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JO - Life Sciences

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SN - 0024-3205

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ER -

Dopamine receptor binding: Specificity, localization and regulation by ions and guanyl nucleotides

Abstract

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