Dopamine receptor binding in the corpus striatum of mammalian brain

Specific binding of [³H]dopamine to membranes from the corpus striatum of rat and calf brain appears to involve the postsynaptic dopamine receptor. Specific [³H]dopamine binding is saturable, with half maximal binding in calf membranes at 7 nM. Apomorphine is about twice as potent as dopamine in competing for binding sites, whereas (-)norepinephrine is 5% as potent as dopamine and isoproterenol is virtually inactive. The relative potencies of phenothiazines as inhibitors of specific dopamine binding correlates with their clinical potencies and actions on the dopamine sensitive adenylate cyclase.