Dopamine D2 and D4 receptor heteromerization and its allosteric receptor-receptor interactions

Dasiel O. Borroto-Escuela; Kathleen Van Craenenbroeck; Wilber Romero-Fernandez; Diego Guidolin; Amina S. Woods; Alicia Rivera; Guy Haegeman; Luigi F. Agnati; Alexander O. Tarakanov; Kjell Fuxe

doi:10.1016/j.bbrc.2010.12.083

Dopamine D2 and D4 receptor heteromerization and its allosteric receptor-receptor interactions

Dasiel O. Borroto-Escuela, Kathleen Van Craenenbroeck, Wilber Romero-Fernandez, Diego Guidolin, Amina S. Woods, Alicia Rivera, Guy Haegeman, Luigi F. Agnati, Alexander O. Tarakanov, Kjell Fuxe

School of Medicine

Research output: Contribution to journal › Article › peer-review

72 Scopus citations

Abstract

Dopamine D₂ and D₄ receptors partially codistribute in the dorsal striatum and appear to play a fundamental role in complex behaviors and motor function. The discovery of D₂R-D_4.xR (D_4.2R, D_4.4R or D_4.7R) heteromers has been made in cellular models using co-immunoprecipitation, in situ Proximity Ligation Assays and BRET¹ techniques with the D₂R and D_4.7R receptors being the least effective in forming heteromers. Allosteric receptor-receptor interactions in D₂R-D_4.2R and D₂R-D_4.4 R heteromers were observed using the MAPK assays indicating the existence of an enhancing allosteric receptor-receptor interaction in the corresponding heteromers between the two orthosteric binding sites. The bioinformatic predictions suggest the existence of a basic set of common triplets (ALQ and LRA) in the two participating receptors that may contribute to the receptor-receptor interaction interfaces.

Original language	English (US)
Pages (from-to)	928-934
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	404
Issue number	4
DOIs	https://doi.org/10.1016/j.bbrc.2010.12.083
State	Published - Jan 28 2011

Keywords

Allosteric modulation
Dopamine DR receptor
Dopamine DR receptor
G protein-coupled receptors
Heteromerization
Protein-protein interactions.

ASJC Scopus subject areas

Biochemistry
Biophysics
Cell Biology
Molecular Biology

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Borroto-Escuela, D. O., Craenenbroeck, K. V., Romero-Fernandez, W., Guidolin, D., Woods, A. S., Rivera, A., Haegeman, G., Agnati, L. F., Tarakanov, A. O., & Fuxe, K. (2011). Dopamine D2 and D4 receptor heteromerization and its allosteric receptor-receptor interactions. Biochemical and Biophysical Research Communications, 404(4), 928-934. https://doi.org/10.1016/j.bbrc.2010.12.083

Borroto-Escuela, DO, Craenenbroeck, KV, Romero-Fernandez, W, Guidolin, D, Woods, AS, Rivera, A, Haegeman, G, Agnati, LF, Tarakanov, AO & Fuxe, K 2011, 'Dopamine D2 and D4 receptor heteromerization and its allosteric receptor-receptor interactions', Biochemical and Biophysical Research Communications, vol. 404, no. 4, pp. 928-934. https://doi.org/10.1016/j.bbrc.2010.12.083

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Dopamine D2 and D4 receptor heteromerization and its allosteric receptor-receptor interactions

Abstract

Keywords

ASJC Scopus subject areas

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