Dopa decarboxylase activity of the living human brain

Albert Gjedde; Jakob Reith; Suzan Dyve; Gabriel Léger; Mark Guttman; Mirko Diksic; Alan Evans; Hiroto Kuwabara

doi:10.1073/pnas.88.7.2721

Dopa decarboxylase activity of the living human brain

Albert Gjedde, Jakob Reith, Suzan Dyve, Gabriel Léger, Mark Guttman, Mirko Diksic, Alan Evans, Hiroto Kuwabara

Research output: Contribution to journal › Article › peer-review

139 Scopus citations

Abstract

Monoaminergic neurons use dopa decarboxylase (DDC; aromatic-L-amino-acid carboxy-lyase, EC 4.1.1.28) to form dopamine from L-3,4-dihydroxyphenylalanine (L-dopa). We measured regional dopa decarboxylase activity in brains of six healthy volunteers with 6-[¹⁸F]fluoro-L-dopa and positron emission tomography. We calculated the enzyme activity, relative to its K_m, with a kinetic model that yielded the relative rate of conversion of 6-[¹⁸F]fluoro-L-dopa to [¹⁸F]fluorodopamine. Regional values of relative dopa decarboxylase activity ranged from nil in occipital cortex to 1.9 h^-1 in caudate nucleus and putamen, in agreement with values obtained in vitro.

Original language	English (US)
Pages (from-to)	2721-2725
Number of pages	5
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	88
Issue number	7
DOIs	https://doi.org/10.1073/pnas.88.7.2721
State	Published - Apr 1 1991
Externally published	Yes

Keywords

6-[F]fluoro-L-dopa
Dopamine
Dopamine synthesis
Parkinson disease
Positron emission tomography

ASJC Scopus subject areas

General

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10.1073/pnas.88.7.2721

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title = "Dopa decarboxylase activity of the living human brain",

abstract = "Monoaminergic neurons use dopa decarboxylase (DDC; aromatic-L-amino-acid carboxy-lyase, EC 4.1.1.28) to form dopamine from L-3,4-dihydroxyphenylalanine (L-dopa). We measured regional dopa decarboxylase activity in brains of six healthy volunteers with 6-[18F]fluoro-L-dopa and positron emission tomography. We calculated the enzyme activity, relative to its Km, with a kinetic model that yielded the relative rate of conversion of 6-[18F]fluoro-L-dopa to [18F]fluorodopamine. Regional values of relative dopa decarboxylase activity ranged from nil in occipital cortex to 1.9 h-1 in caudate nucleus and putamen, in agreement with values obtained in vitro.",

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author = "Albert Gjedde and Jakob Reith and Suzan Dyve and Gabriel L{\'e}ger and Mark Guttman and Mirko Diksic and Alan Evans and Hiroto Kuwabara",

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T1 - Dopa decarboxylase activity of the living human brain

AU - Gjedde, Albert

AU - Reith, Jakob

AU - Dyve, Suzan

AU - Léger, Gabriel

AU - Guttman, Mark

AU - Diksic, Mirko

AU - Evans, Alan

AU - Kuwabara, Hiroto

PY - 1991/4/1

Y1 - 1991/4/1

N2 - Monoaminergic neurons use dopa decarboxylase (DDC; aromatic-L-amino-acid carboxy-lyase, EC 4.1.1.28) to form dopamine from L-3,4-dihydroxyphenylalanine (L-dopa). We measured regional dopa decarboxylase activity in brains of six healthy volunteers with 6-[18F]fluoro-L-dopa and positron emission tomography. We calculated the enzyme activity, relative to its Km, with a kinetic model that yielded the relative rate of conversion of 6-[18F]fluoro-L-dopa to [18F]fluorodopamine. Regional values of relative dopa decarboxylase activity ranged from nil in occipital cortex to 1.9 h-1 in caudate nucleus and putamen, in agreement with values obtained in vitro.

AB - Monoaminergic neurons use dopa decarboxylase (DDC; aromatic-L-amino-acid carboxy-lyase, EC 4.1.1.28) to form dopamine from L-3,4-dihydroxyphenylalanine (L-dopa). We measured regional dopa decarboxylase activity in brains of six healthy volunteers with 6-[18F]fluoro-L-dopa and positron emission tomography. We calculated the enzyme activity, relative to its Km, with a kinetic model that yielded the relative rate of conversion of 6-[18F]fluoro-L-dopa to [18F]fluorodopamine. Regional values of relative dopa decarboxylase activity ranged from nil in occipital cortex to 1.9 h-1 in caudate nucleus and putamen, in agreement with values obtained in vitro.

KW - 6-[F]fluoro-L-dopa

KW - Dopamine

KW - Dopamine synthesis

KW - Parkinson disease

KW - Positron emission tomography

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JF - Proceedings of the National Academy of Sciences of the United States of America

IS - 7

ER -

Dopa decarboxylase activity of the living human brain

Abstract

Keywords

ASJC Scopus subject areas

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