Donor cell type can influence the epigenome and differentiation potential of human induced pluripotent stem cells

Kitai Kim, Rui Zhao, Akiko Doi, Kitwa Ng, Juli Unternaehrer, Patrick Cahan, Huo Hongguang, Yuin Han Loh, Martin J. Aryee, M. William Lensch, Hu Li, James J. Collins, Andrew P Feinberg, George Q. Daley

Research output: Contribution to journalArticle

Abstract

We compared bona fide human induced pluripotent stem cells (iPSCs) derived from umbilical cord blood (CB) cells and neonatal keratinocytes (K). As a consequence of both incomplete erasure of tissue-specific methylation and aberrant de novo methylation, CB-iPSCs and K-iPSCs were distinct in genome-wide DNA methylation profiles and differentiation potential. Extended passage of some iPSC clones in culture did not improve their epigenetic resemblance to embryonic stem cells, implying that some human iPSCs retain a residual 'epigenetic memory' of their tissue of origin.

Original languageEnglish (US)
Pages (from-to)1117-1119
Number of pages3
JournalNature Biotechnology
Volume29
Issue number12
DOIs
StatePublished - Dec 2011

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Induced Pluripotent Stem Cells
Stem cells
Methylation
Fetal Blood
Epigenomics
Blood
Tissue
DNA Methylation
Embryonic Stem Cells
Keratinocytes
Blood Cells
Cell culture
Clone Cells
Genome
Genes
Cells
Data storage equipment

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Biotechnology
  • Molecular Medicine
  • Bioengineering
  • Biomedical Engineering

Cite this

Donor cell type can influence the epigenome and differentiation potential of human induced pluripotent stem cells. / Kim, Kitai; Zhao, Rui; Doi, Akiko; Ng, Kitwa; Unternaehrer, Juli; Cahan, Patrick; Hongguang, Huo; Loh, Yuin Han; Aryee, Martin J.; Lensch, M. William; Li, Hu; Collins, James J.; Feinberg, Andrew P; Daley, George Q.

In: Nature Biotechnology, Vol. 29, No. 12, 12.2011, p. 1117-1119.

Research output: Contribution to journalArticle

Kim, K, Zhao, R, Doi, A, Ng, K, Unternaehrer, J, Cahan, P, Hongguang, H, Loh, YH, Aryee, MJ, Lensch, MW, Li, H, Collins, JJ, Feinberg, AP & Daley, GQ 2011, 'Donor cell type can influence the epigenome and differentiation potential of human induced pluripotent stem cells', Nature Biotechnology, vol. 29, no. 12, pp. 1117-1119. https://doi.org/10.1038/nbt.2052
Kim, Kitai ; Zhao, Rui ; Doi, Akiko ; Ng, Kitwa ; Unternaehrer, Juli ; Cahan, Patrick ; Hongguang, Huo ; Loh, Yuin Han ; Aryee, Martin J. ; Lensch, M. William ; Li, Hu ; Collins, James J. ; Feinberg, Andrew P ; Daley, George Q. / Donor cell type can influence the epigenome and differentiation potential of human induced pluripotent stem cells. In: Nature Biotechnology. 2011 ; Vol. 29, No. 12. pp. 1117-1119.
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