TY - JOUR
T1 - Domestic pig (Sus scrofa) as an animal model for experimental Trypanosoma cruzi infection
AU - Yauri, Verónica
AU - Castro-Sesquen, Yagahira E.
AU - Verastegui, Manuela
AU - Angulo, Noelia
AU - Recuenco, Fernando
AU - Cabello, Ines
AU - Malaga, Edith
AU - Bern, Caryn
AU - Gavidia, Cesar M.
AU - Gilman, Robert H.
N1 - Funding Information:
This project was funded by Fogarty International Center (5 R24 TW007988, D43 TW006581) and National Institute of Allergy and Infectious Diseases (R01 AI087776) at the National Institutes of Health. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Publisher Copyright:
Copyright © 2016 by The American Society of Tropical Medicine and Hygiene.
PY - 2016/5
Y1 - 2016/5
N2 - Pigs were infected with a Bolivian strain of Trypanosoma cruzi (genotype I) and evaluated up to 150 days postinoculation (dpi) to determine the use of pigs as an animal model of Chagas disease. Parasitemia was observed in the infected pigs during the acute phase (15-40 dpi). Anti-T.cruzi immunoglobulin M was detected during 15-75 dpi; high levels of anti-T.cruzi immunoglobulin G were detected in all infected pigs from 75 to 150 dpi. Parasitic DNA was observed by western blot (58%, 28/48) and polymerase chain reaction (27%, 13/48) in urine samples, and in the brain (75%, 3/4), spleen (50%, 2/4), and duodenum (25%, 1/4), but no parasitic DNA was found in the heart, colon, and kidney. Parasites were not observed microscopically in tissues samples, but mild inflammation, vasculitis, and congestion was observed in heart, brain, kidney, and spleen. This pig model was useful for the standardization of the urine test because of the higher volume that can be obtained as compared with other small animal models. However, further experiments are required to observe pathological changes characteristic of Chagas disease in humans.
AB - Pigs were infected with a Bolivian strain of Trypanosoma cruzi (genotype I) and evaluated up to 150 days postinoculation (dpi) to determine the use of pigs as an animal model of Chagas disease. Parasitemia was observed in the infected pigs during the acute phase (15-40 dpi). Anti-T.cruzi immunoglobulin M was detected during 15-75 dpi; high levels of anti-T.cruzi immunoglobulin G were detected in all infected pigs from 75 to 150 dpi. Parasitic DNA was observed by western blot (58%, 28/48) and polymerase chain reaction (27%, 13/48) in urine samples, and in the brain (75%, 3/4), spleen (50%, 2/4), and duodenum (25%, 1/4), but no parasitic DNA was found in the heart, colon, and kidney. Parasites were not observed microscopically in tissues samples, but mild inflammation, vasculitis, and congestion was observed in heart, brain, kidney, and spleen. This pig model was useful for the standardization of the urine test because of the higher volume that can be obtained as compared with other small animal models. However, further experiments are required to observe pathological changes characteristic of Chagas disease in humans.
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U2 - 10.4269/ajtmh.15-0233
DO - 10.4269/ajtmh.15-0233
M3 - Article
C2 - 26928841
AN - SCOPUS:84966658484
SN - 0002-9637
VL - 94
SP - 1020
EP - 1027
JO - American Journal of Tropical Medicine and Hygiene
JF - American Journal of Tropical Medicine and Hygiene
IS - 5
ER -