Domains involved in the in vivo function and oligomerization of apical growth determinant DivIVA in Streptomyces coelicolor

Shengbing Wang, Stuart Cantlay, Niklas Nordberg, Michal Letek, José A. Gil, Klas Flärdh

Research output: Contribution to journalArticle

Abstract

The coiled-coil protein DivIVA is a determinant of apical growth and hyphal branching in Streptomyces coelicolor. We have investigated the properties of this protein and the involvement of different domains in its essential function and subcellular targeting. In S. coelicolor cell extracts, DivIVA was present as large oligomeric complexes that were not strongly membrane associated. The purified protein could self-assemble into extensive protein filaments in vitro. Two large and conspicuous segments in the amino acid sequence of streptomycete DivIVAs not present in other homologs, an internal PQG-rich segment and a carboxy-terminal extension, are shown to be dispensable for the essential function in S. coelicolor. Instead, the highly conserved amino-terminal of 22 amino acids was required and affected establishment of new DivIVA foci and hyphal branches, and an essential coiled-coil domain affected oligomerization of the protein.

Original languageEnglish (US)
Pages (from-to)100-109
Number of pages10
JournalFEMS Microbiology Letters
Volume297
Issue number1
DOIs
StatePublished - Aug 2009
Externally publishedYes

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Streptomyces coelicolor
Growth
Proteins
Cell Extracts
Amino Acid Sequence
Amino Acids
Membranes

Keywords

  • Apical growth
  • Cell polarity
  • Coiled coil
  • DivIVA
  • Hyphae
  • Streptomyces

ASJC Scopus subject areas

  • Microbiology
  • Genetics
  • Molecular Biology

Cite this

Domains involved in the in vivo function and oligomerization of apical growth determinant DivIVA in Streptomyces coelicolor. / Wang, Shengbing; Cantlay, Stuart; Nordberg, Niklas; Letek, Michal; Gil, José A.; Flärdh, Klas.

In: FEMS Microbiology Letters, Vol. 297, No. 1, 08.2009, p. 100-109.

Research output: Contribution to journalArticle

Wang, Shengbing ; Cantlay, Stuart ; Nordberg, Niklas ; Letek, Michal ; Gil, José A. ; Flärdh, Klas. / Domains involved in the in vivo function and oligomerization of apical growth determinant DivIVA in Streptomyces coelicolor. In: FEMS Microbiology Letters. 2009 ; Vol. 297, No. 1. pp. 100-109.
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