Dom34

Hbs1 promotes subunit dissociation and peptidyl-tRNA drop-off to initiate no-go decay

Christopher J. Shoemaker, Daniel E. Eyler, Rachel Green

Research output: Contribution to journalArticle

Abstract

No-go decay (NGD) is one of several messenger RNA (mRNA) surveillance systems dedicated to the removal of defective mRNAs from the available pool. Two interacting factors, Dom34 and Hbs1, are genetically implicated in NGD in yeast. Using a reconstituted yeast translation system, we show that Dom34:Hbs1 interacts with the ribosome to promote subunit dissociation and peptidyl-tRNA drop-off. Our data further indicate that these recycling activities are shared by the homologous translation termination factor complex eRF1:eRF3, suggesting a common ancestral function. Because Dom34:Hbs1 activity exhibits no dependence on either peptide length or A-site codon identity, we propose that this quality-control system functions broadly to recycle ribosomes throughout the translation cycle whenever stalls occur.

Original languageEnglish (US)
Pages (from-to)369-372
Number of pages4
JournalScience
Volume330
Issue number6002
DOIs
StatePublished - Oct 15 2010

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Ribosomes
Yeasts
Messenger RNA
Recycling
Codon
Quality Control
Peptides
peptidyl-tRNA

ASJC Scopus subject areas

  • General
  • Medicine(all)

Cite this

Dom34 : Hbs1 promotes subunit dissociation and peptidyl-tRNA drop-off to initiate no-go decay. / Shoemaker, Christopher J.; Eyler, Daniel E.; Green, Rachel.

In: Science, Vol. 330, No. 6002, 15.10.2010, p. 369-372.

Research output: Contribution to journalArticle

Shoemaker, Christopher J. ; Eyler, Daniel E. ; Green, Rachel. / Dom34 : Hbs1 promotes subunit dissociation and peptidyl-tRNA drop-off to initiate no-go decay. In: Science. 2010 ; Vol. 330, No. 6002. pp. 369-372.
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