Does the cryogenic freezing process cause shorter telomeres?

Edmund C. Jenkins, Lingling Ye, Wayne P. Silverman

Research output: Contribution to journalArticlepeer-review

Abstract

We have observed evidence of increased telomere shortening in short-term T-lymphocyte cultures following freezing and thawing of the original inoculum obtained by ficoll-paque gradient centrifugation, compared to T-lymphocytes that were cultured immediately without freezing and thawing from the same blood sample from 3 female and 3 male adults. Because freezing may have similar effects on other cell types, and because telomere shortening may only manifest its effects after many years or decades, we suggest there is a pressing need for evaluation of the effects of freezing on any cells envisioned for clinical applications, including embryo implantation.

Original languageEnglish (US)
Pages (from-to)72-73
Number of pages2
JournalCryobiology
Volume65
Issue number1
DOIs
StatePublished - Aug 2012

Keywords

  • Cryogenics
  • Embryos
  • FISH
  • PNA probe
  • Short-term cultures
  • Stem cells
  • T-lymphocytes
  • Telomere " loss"
  • Telomere shortening

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

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