Does Adiponectin Upregulation Attenuate the Severity of Acute Pancreatitis in Obesity?

Hayder H. Al-Azzawi, Kathryn M. Ziegler, Deborah A. Swartz-Basile, Sue Wang, Henry A. Pitt, Nicholas J. Zyromski

Research output: Contribution to journalArticle

Abstract

Introduction: Obesity is an independent risk factor for severe acute pancreatitis, though the mechanisms underlying this association are unknown. The powerful anti-inflammatory adipokine adiponectin is decreased in obesity. We recently showed that the severity of pancreatitis in obese mice is inversely related to circulating adiponectin levels, and therefore hypothesized that adiponectin upregulation would attenuate the severity of pancreatitis in obese mice. Methods: Forty congenitally obese mice were studied. Seven days prior to study, 20 mice received a single tail vein injection of adenovirus expressing recombinant murine adiponectin (APN; 2 × 10 8 plaque forming unit (pfu)), and the remainder received a control adenoviral vector expressing β-galactosidase (β-gal; 2 × 10 8 pfu). Half of the mice in each group had pancreatitis induced by cerulein injection (50mcg/kg IP hourly for 6 h). The other half received saline on the same schedule. Serum APN concentration and pancreatic tissue concentrations of interleukin (IL)-6, IL-1β, and MCP-1 were measured by ELISA. Histologic pancreatitis score was calculated based on the degree of inflammation (0-4), edema (0-4), and vacuolization (0-4). Data were analyzed by ANOVA and Tukey's tests; p

Original languageEnglish (US)
Pages (from-to)1394-1400
Number of pages7
JournalJournal of Gastrointestinal Surgery
Volume15
Issue number8
DOIs
StatePublished - Aug 2011
Externally publishedYes

    Fingerprint

Keywords

  • Adenovirus
  • Adipokine
  • Adiponectin
  • Obesity
  • Pancreatitis

ASJC Scopus subject areas

  • Surgery
  • Gastroenterology

Cite this

Al-Azzawi, H. H., Ziegler, K. M., Swartz-Basile, D. A., Wang, S., Pitt, H. A., & Zyromski, N. J. (2011). Does Adiponectin Upregulation Attenuate the Severity of Acute Pancreatitis in Obesity? Journal of Gastrointestinal Surgery, 15(8), 1394-1400. https://doi.org/10.1007/s11605-011-1583-x