TY - JOUR
T1 - Does Adiponectin Upregulation Attenuate the Severity of Acute Pancreatitis in Obesity?
AU - Al-Azzawi, Hayder H.
AU - Ziegler, Kathryn M.
AU - Swartz-Basile, Deborah A.
AU - Wang, Sue
AU - Pitt, Henry A.
AU - Zyromski, Nicholas J.
N1 - Funding Information:
Presented at the Digestive Disease Week (DDW) (SSAT) meeting, Chicago, IL, 30 May–4 June 2009 Supported by the Society for Surgery of the Alimentary Tract (SSAT) Career Development Award (Nicholas J. Zyromski) H.H.Al-Azzawi.K.M.Ziegler.D.A.Swartz-Basile. S. Wang.H. A. Pitt.N. J. Zyromski (*) Department of Surgery, Indiana University School of Medicine, 535 Barnhill Drive, RT 130, Indianapolis, IN 46202, USA e-mail: nzyromsk@iupui.edu
Copyright:
Copyright 2012 Elsevier B.V., All rights reserved.
PY - 2011/8
Y1 - 2011/8
N2 - Introduction: Obesity is an independent risk factor for severe acute pancreatitis, though the mechanisms underlying this association are unknown. The powerful anti-inflammatory adipokine adiponectin is decreased in obesity. We recently showed that the severity of pancreatitis in obese mice is inversely related to circulating adiponectin levels, and therefore hypothesized that adiponectin upregulation would attenuate the severity of pancreatitis in obese mice. Methods: Forty congenitally obese mice were studied. Seven days prior to study, 20 mice received a single tail vein injection of adenovirus expressing recombinant murine adiponectin (APN; 2 × 10 8 plaque forming unit (pfu)), and the remainder received a control adenoviral vector expressing β-galactosidase (β-gal; 2 × 10 8 pfu). Half of the mice in each group had pancreatitis induced by cerulein injection (50mcg/kg IP hourly for 6 h). The other half received saline on the same schedule. Serum APN concentration and pancreatic tissue concentrations of interleukin (IL)-6, IL-1β, and MCP-1 were measured by ELISA. Histologic pancreatitis score was calculated based on the degree of inflammation (0-4), edema (0-4), and vacuolization (0-4). Data were analyzed by ANOVA and Tukey's tests; p<0.05 was considered significant. Results: No difference in body weight was observed between groups. Serum APN was significantly upregulated in the APN group compared with the β-gal group. Pancreatic tissue concentration of IL-6 was significantly decreased in the APN group compared with the β-gal group. No change either in pancreatic tissue concentration of IL-1β and MCP-1 or in the severity of histologic pancreatitis were observed. Conclusion: Adiponectin upregulation modulates the pancreatic cytokine milieu but does not attenuate pancreatitis in this model of mild acute pancreatitis.
AB - Introduction: Obesity is an independent risk factor for severe acute pancreatitis, though the mechanisms underlying this association are unknown. The powerful anti-inflammatory adipokine adiponectin is decreased in obesity. We recently showed that the severity of pancreatitis in obese mice is inversely related to circulating adiponectin levels, and therefore hypothesized that adiponectin upregulation would attenuate the severity of pancreatitis in obese mice. Methods: Forty congenitally obese mice were studied. Seven days prior to study, 20 mice received a single tail vein injection of adenovirus expressing recombinant murine adiponectin (APN; 2 × 10 8 plaque forming unit (pfu)), and the remainder received a control adenoviral vector expressing β-galactosidase (β-gal; 2 × 10 8 pfu). Half of the mice in each group had pancreatitis induced by cerulein injection (50mcg/kg IP hourly for 6 h). The other half received saline on the same schedule. Serum APN concentration and pancreatic tissue concentrations of interleukin (IL)-6, IL-1β, and MCP-1 were measured by ELISA. Histologic pancreatitis score was calculated based on the degree of inflammation (0-4), edema (0-4), and vacuolization (0-4). Data were analyzed by ANOVA and Tukey's tests; p<0.05 was considered significant. Results: No difference in body weight was observed between groups. Serum APN was significantly upregulated in the APN group compared with the β-gal group. Pancreatic tissue concentration of IL-6 was significantly decreased in the APN group compared with the β-gal group. No change either in pancreatic tissue concentration of IL-1β and MCP-1 or in the severity of histologic pancreatitis were observed. Conclusion: Adiponectin upregulation modulates the pancreatic cytokine milieu but does not attenuate pancreatitis in this model of mild acute pancreatitis.
KW - Adenovirus
KW - Adipokine
KW - Adiponectin
KW - Obesity
KW - Pancreatitis
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U2 - 10.1007/s11605-011-1583-x
DO - 10.1007/s11605-011-1583-x
M3 - Article
C2 - 21660639
AN - SCOPUS:79960845606
VL - 15
SP - 1394
EP - 1400
JO - Journal of Gastrointestinal Surgery
JF - Journal of Gastrointestinal Surgery
SN - 1091-255X
IS - 8
ER -