Does Adiponectin Upregulation Attenuate the Severity of Acute Pancreatitis in Obesity?

Hayder H. Al-Azzawi; Kathryn M. Ziegler; Deborah A. Swartz-Basile; Sue Wang; Henry A. Pitt; Nicholas J. Zyromski

doi:10.1007/s11605-011-1583-x

Does Adiponectin Upregulation Attenuate the Severity of Acute Pancreatitis in Obesity?

Hayder H. Al-Azzawi, Kathryn M. Ziegler, Deborah A. Swartz-Basile, Sue Wang, Henry A. Pitt, Nicholas J. Zyromski

Research output: Contribution to journal › Article

Abstract

Introduction: Obesity is an independent risk factor for severe acute pancreatitis, though the mechanisms underlying this association are unknown. The powerful anti-inflammatory adipokine adiponectin is decreased in obesity. We recently showed that the severity of pancreatitis in obese mice is inversely related to circulating adiponectin levels, and therefore hypothesized that adiponectin upregulation would attenuate the severity of pancreatitis in obese mice. Methods: Forty congenitally obese mice were studied. Seven days prior to study, 20 mice received a single tail vein injection of adenovirus expressing recombinant murine adiponectin (APN; 2 × 10 ⁸ plaque forming unit (pfu)), and the remainder received a control adenoviral vector expressing β-galactosidase (β-gal; 2 × 10 ⁸ pfu). Half of the mice in each group had pancreatitis induced by cerulein injection (50mcg/kg IP hourly for 6 h). The other half received saline on the same schedule. Serum APN concentration and pancreatic tissue concentrations of interleukin (IL)-6, IL-1β, and MCP-1 were measured by ELISA. Histologic pancreatitis score was calculated based on the degree of inflammation (0-4), edema (0-4), and vacuolization (0-4). Data were analyzed by ANOVA and Tukey's tests; p

Original language	English (US)
Pages (from-to)	1394-1400
Number of pages	7
Journal	Journal of Gastrointestinal Surgery
Volume	15
Issue number	8
DOIs	https://doi.org/10.1007/s11605-011-1583-x
State	Published - Aug 2011
Externally published	Yes

Fingerprint

Adiponectin

Pancreatitis

Up-Regulation

Obesity

Obese Mice

Galactosidases

Ceruletide

Injections

Adipokines

Interleukin-1

Adenoviridae

Tail

Veins

Interleukin-6

Edema

Analysis of Variance

Appointments and Schedules

Anti-Inflammatory Agents

Enzyme-Linked Immunosorbent Assay

Inflammation

Keywords

Adenovirus
Adipokine
Adiponectin
Obesity
Pancreatitis

ASJC Scopus subject areas

Surgery
Gastroenterology

Cite this

Al-Azzawi, H. H., Ziegler, K. M., Swartz-Basile, D. A., Wang, S., Pitt, H. A., & Zyromski, N. J. (2011). Does Adiponectin Upregulation Attenuate the Severity of Acute Pancreatitis in Obesity? Journal of Gastrointestinal Surgery, 15(8), 1394-1400. https://doi.org/10.1007/s11605-011-1583-x

Does Adiponectin Upregulation Attenuate the Severity of Acute Pancreatitis in Obesity? / Al-Azzawi, Hayder H.; Ziegler, Kathryn M.; Swartz-Basile, Deborah A.; Wang, Sue; Pitt, Henry A.; Zyromski, Nicholas J.

In: Journal of Gastrointestinal Surgery, Vol. 15, No. 8, 08.2011, p. 1394-1400.

Research output: Contribution to journal › Article

Al-Azzawi, HH, Ziegler, KM, Swartz-Basile, DA, Wang, S, Pitt, HA & Zyromski, NJ 2011, 'Does Adiponectin Upregulation Attenuate the Severity of Acute Pancreatitis in Obesity?', Journal of Gastrointestinal Surgery, vol. 15, no. 8, pp. 1394-1400. https://doi.org/10.1007/s11605-011-1583-x

Al-Azzawi HH, Ziegler KM, Swartz-Basile DA, Wang S, Pitt HA, Zyromski NJ. Does Adiponectin Upregulation Attenuate the Severity of Acute Pancreatitis in Obesity? Journal of Gastrointestinal Surgery. 2011 Aug;15(8):1394-1400. https://doi.org/10.1007/s11605-011-1583-x

Al-Azzawi, Hayder H. ; Ziegler, Kathryn M. ; Swartz-Basile, Deborah A. ; Wang, Sue ; Pitt, Henry A. ; Zyromski, Nicholas J. / Does Adiponectin Upregulation Attenuate the Severity of Acute Pancreatitis in Obesity?. In: Journal of Gastrointestinal Surgery. 2011 ; Vol. 15, No. 8. pp. 1394-1400.

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title = "Does Adiponectin Upregulation Attenuate the Severity of Acute Pancreatitis in Obesity?",

abstract = "Introduction: Obesity is an independent risk factor for severe acute pancreatitis, though the mechanisms underlying this association are unknown. The powerful anti-inflammatory adipokine adiponectin is decreased in obesity. We recently showed that the severity of pancreatitis in obese mice is inversely related to circulating adiponectin levels, and therefore hypothesized that adiponectin upregulation would attenuate the severity of pancreatitis in obese mice. Methods: Forty congenitally obese mice were studied. Seven days prior to study, 20 mice received a single tail vein injection of adenovirus expressing recombinant murine adiponectin (APN; 2 × 10 8 plaque forming unit (pfu)), and the remainder received a control adenoviral vector expressing β-galactosidase (β-gal; 2 × 10 8 pfu). Half of the mice in each group had pancreatitis induced by cerulein injection (50mcg/kg IP hourly for 6 h). The other half received saline on the same schedule. Serum APN concentration and pancreatic tissue concentrations of interleukin (IL)-6, IL-1β, and MCP-1 were measured by ELISA. Histologic pancreatitis score was calculated based on the degree of inflammation (0-4), edema (0-4), and vacuolization (0-4). Data were analyzed by ANOVA and Tukey's tests; p",

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author = "Al-Azzawi, {Hayder H.} and Ziegler, {Kathryn M.} and Swartz-Basile, {Deborah A.} and Sue Wang and Pitt, {Henry A.} and Zyromski, {Nicholas J.}",

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