DNAJB1-PRKACA is specific for fibrolamellar carcinoma

Rondell P. Graham, Long Jin, Darlene L. Knutson, Sara M. Kloft-Nelson, Patricia T. Greipp, Nina Waldburger, Stephanie Roessler, Thomas Longerich, Lewis R. Roberts, Andre M. Oliveira, Kevin C. Halling, Peter Schirmacher, Michael S. Torbenson

Research output: Contribution to journalArticle

Abstract

Fibrolamellar carcinoma is a distinct subtype of hepatocellular carcinoma that predominantly affects young patients without underlying cirrhosis. A recurrent DNAJB1-PRKACA fusion has recently been reported in fibrolamellar carcinomas. To determine the specificity of this fusion and to develop routinely available clinical methods of detection, we developed an RT-PCR assay for paraffin-embedded tissues and a FISH probe for detection of the rearrangements of the PRKACA locus. We also developed an RNA in situ hybridization assay to assess expression levels of the total chimeric transcript and wild-type transcripts. A total of 106 primary liver tumors were studied by RT-PCR, including 26 fibrolamellar carcinomas (4 of which were metastases to the abdominal wall or lymph nodes), 25 conventional hepatocellular carcinomas, 25 cholangiocarcinomas, 25 hepatic adenomas, and 5 hepatoblastomas. RT-PCR was successful in 92% of tested fibrolamellar carcinoma cases (24/26) and the DNAJB1-PRKACA fusion transcript was found in all fibrolamellar carcinomas but not in other tumor types. FISH was tested in 19 fibrolamellar carcinomas and in 6 scirrhous hepatocellular carcinomas, which can closely mimic fibrolamellar carcinoma. Rearrangements of the PRKACA locus was seen in all 19 fibrolamellar carcinoma specimens, but in none of the scirrhous hepatocellular carcinomas. Finally, a RNA in situ hybridization strategy was positive in 7/7 successfully hybridized cases, and showed mRNA over-expression in all of the fibrolamellar carcinomas. In addition, the stromal cells embedded in the characteristic intratumoral fibrosis of fibrolamellar carcinomas and the background liver tissues were negative for the DNAJB1-PRKACA fusion by all tested methods. In conclusion, detection of DNAJB1-PRKACA is a very sensitive and specific finding in support of the diagnosis of fibrolamellar carcinoma.

Original languageEnglish (US)
Pages (from-to)822-829
Number of pages8
JournalModern Pathology
Volume28
Issue number6
DOIs
StatePublished - Jun 1 2015
Externally publishedYes

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Carcinoma
Hepatocellular Carcinoma
Scirrhous Adenocarcinoma
Polymerase Chain Reaction
In Situ Hybridization
Liver
Fibrosis
RNA
Hepatoblastoma
Cholangiocarcinoma
Abdominal Wall
Stromal Cells
Adenoma
Paraffin
Neoplasms
Lymph Nodes
Neoplasm Metastasis
Messenger RNA

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Graham, R. P., Jin, L., Knutson, D. L., Kloft-Nelson, S. M., Greipp, P. T., Waldburger, N., ... Torbenson, M. S. (2015). DNAJB1-PRKACA is specific for fibrolamellar carcinoma. Modern Pathology, 28(6), 822-829. https://doi.org/10.1038/modpathol.2015.4

DNAJB1-PRKACA is specific for fibrolamellar carcinoma. / Graham, Rondell P.; Jin, Long; Knutson, Darlene L.; Kloft-Nelson, Sara M.; Greipp, Patricia T.; Waldburger, Nina; Roessler, Stephanie; Longerich, Thomas; Roberts, Lewis R.; Oliveira, Andre M.; Halling, Kevin C.; Schirmacher, Peter; Torbenson, Michael S.

In: Modern Pathology, Vol. 28, No. 6, 01.06.2015, p. 822-829.

Research output: Contribution to journalArticle

Graham, RP, Jin, L, Knutson, DL, Kloft-Nelson, SM, Greipp, PT, Waldburger, N, Roessler, S, Longerich, T, Roberts, LR, Oliveira, AM, Halling, KC, Schirmacher, P & Torbenson, MS 2015, 'DNAJB1-PRKACA is specific for fibrolamellar carcinoma', Modern Pathology, vol. 28, no. 6, pp. 822-829. https://doi.org/10.1038/modpathol.2015.4
Graham RP, Jin L, Knutson DL, Kloft-Nelson SM, Greipp PT, Waldburger N et al. DNAJB1-PRKACA is specific for fibrolamellar carcinoma. Modern Pathology. 2015 Jun 1;28(6):822-829. https://doi.org/10.1038/modpathol.2015.4
Graham, Rondell P. ; Jin, Long ; Knutson, Darlene L. ; Kloft-Nelson, Sara M. ; Greipp, Patricia T. ; Waldburger, Nina ; Roessler, Stephanie ; Longerich, Thomas ; Roberts, Lewis R. ; Oliveira, Andre M. ; Halling, Kevin C. ; Schirmacher, Peter ; Torbenson, Michael S. / DNAJB1-PRKACA is specific for fibrolamellar carcinoma. In: Modern Pathology. 2015 ; Vol. 28, No. 6. pp. 822-829.
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abstract = "Fibrolamellar carcinoma is a distinct subtype of hepatocellular carcinoma that predominantly affects young patients without underlying cirrhosis. A recurrent DNAJB1-PRKACA fusion has recently been reported in fibrolamellar carcinomas. To determine the specificity of this fusion and to develop routinely available clinical methods of detection, we developed an RT-PCR assay for paraffin-embedded tissues and a FISH probe for detection of the rearrangements of the PRKACA locus. We also developed an RNA in situ hybridization assay to assess expression levels of the total chimeric transcript and wild-type transcripts. A total of 106 primary liver tumors were studied by RT-PCR, including 26 fibrolamellar carcinomas (4 of which were metastases to the abdominal wall or lymph nodes), 25 conventional hepatocellular carcinomas, 25 cholangiocarcinomas, 25 hepatic adenomas, and 5 hepatoblastomas. RT-PCR was successful in 92{\%} of tested fibrolamellar carcinoma cases (24/26) and the DNAJB1-PRKACA fusion transcript was found in all fibrolamellar carcinomas but not in other tumor types. FISH was tested in 19 fibrolamellar carcinomas and in 6 scirrhous hepatocellular carcinomas, which can closely mimic fibrolamellar carcinoma. Rearrangements of the PRKACA locus was seen in all 19 fibrolamellar carcinoma specimens, but in none of the scirrhous hepatocellular carcinomas. Finally, a RNA in situ hybridization strategy was positive in 7/7 successfully hybridized cases, and showed mRNA over-expression in all of the fibrolamellar carcinomas. In addition, the stromal cells embedded in the characteristic intratumoral fibrosis of fibrolamellar carcinomas and the background liver tissues were negative for the DNAJB1-PRKACA fusion by all tested methods. In conclusion, detection of DNAJB1-PRKACA is a very sensitive and specific finding in support of the diagnosis of fibrolamellar carcinoma.",
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AU - Jin, Long

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AU - Waldburger, Nina

AU - Roessler, Stephanie

AU - Longerich, Thomas

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AU - Oliveira, Andre M.

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N2 - Fibrolamellar carcinoma is a distinct subtype of hepatocellular carcinoma that predominantly affects young patients without underlying cirrhosis. A recurrent DNAJB1-PRKACA fusion has recently been reported in fibrolamellar carcinomas. To determine the specificity of this fusion and to develop routinely available clinical methods of detection, we developed an RT-PCR assay for paraffin-embedded tissues and a FISH probe for detection of the rearrangements of the PRKACA locus. We also developed an RNA in situ hybridization assay to assess expression levels of the total chimeric transcript and wild-type transcripts. A total of 106 primary liver tumors were studied by RT-PCR, including 26 fibrolamellar carcinomas (4 of which were metastases to the abdominal wall or lymph nodes), 25 conventional hepatocellular carcinomas, 25 cholangiocarcinomas, 25 hepatic adenomas, and 5 hepatoblastomas. RT-PCR was successful in 92% of tested fibrolamellar carcinoma cases (24/26) and the DNAJB1-PRKACA fusion transcript was found in all fibrolamellar carcinomas but not in other tumor types. FISH was tested in 19 fibrolamellar carcinomas and in 6 scirrhous hepatocellular carcinomas, which can closely mimic fibrolamellar carcinoma. Rearrangements of the PRKACA locus was seen in all 19 fibrolamellar carcinoma specimens, but in none of the scirrhous hepatocellular carcinomas. Finally, a RNA in situ hybridization strategy was positive in 7/7 successfully hybridized cases, and showed mRNA over-expression in all of the fibrolamellar carcinomas. In addition, the stromal cells embedded in the characteristic intratumoral fibrosis of fibrolamellar carcinomas and the background liver tissues were negative for the DNAJB1-PRKACA fusion by all tested methods. In conclusion, detection of DNAJB1-PRKACA is a very sensitive and specific finding in support of the diagnosis of fibrolamellar carcinoma.

AB - Fibrolamellar carcinoma is a distinct subtype of hepatocellular carcinoma that predominantly affects young patients without underlying cirrhosis. A recurrent DNAJB1-PRKACA fusion has recently been reported in fibrolamellar carcinomas. To determine the specificity of this fusion and to develop routinely available clinical methods of detection, we developed an RT-PCR assay for paraffin-embedded tissues and a FISH probe for detection of the rearrangements of the PRKACA locus. We also developed an RNA in situ hybridization assay to assess expression levels of the total chimeric transcript and wild-type transcripts. A total of 106 primary liver tumors were studied by RT-PCR, including 26 fibrolamellar carcinomas (4 of which were metastases to the abdominal wall or lymph nodes), 25 conventional hepatocellular carcinomas, 25 cholangiocarcinomas, 25 hepatic adenomas, and 5 hepatoblastomas. RT-PCR was successful in 92% of tested fibrolamellar carcinoma cases (24/26) and the DNAJB1-PRKACA fusion transcript was found in all fibrolamellar carcinomas but not in other tumor types. FISH was tested in 19 fibrolamellar carcinomas and in 6 scirrhous hepatocellular carcinomas, which can closely mimic fibrolamellar carcinoma. Rearrangements of the PRKACA locus was seen in all 19 fibrolamellar carcinoma specimens, but in none of the scirrhous hepatocellular carcinomas. Finally, a RNA in situ hybridization strategy was positive in 7/7 successfully hybridized cases, and showed mRNA over-expression in all of the fibrolamellar carcinomas. In addition, the stromal cells embedded in the characteristic intratumoral fibrosis of fibrolamellar carcinomas and the background liver tissues were negative for the DNAJB1-PRKACA fusion by all tested methods. In conclusion, detection of DNAJB1-PRKACA is a very sensitive and specific finding in support of the diagnosis of fibrolamellar carcinoma.

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