DNA TYPING FOR HLA‐DPB1‐ALLELES IN GERMAN PATIENTS WITH SYSTEMIC LUPUS ERYTHEMATOSUS USING THE POLYMERASE CHAIN REACTION AND DIG‐ddUTP‐LABELLED OLIGONUCLEOTIDE PROBES

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Abstract

Genomic DNA of 178 German Caucasian patients with systemic lupus erythematosus are studied for HLA‐DP locus by using PCR and DIG‐ddUTP‐labelled oligonucleotide probes. A significant increase of DPB1*0101 is observed in SLE patients compared with healthy controls (χ < 2 < = 15.27, p.c. <0.004). DPB1*0501 and *0901 are also slightly increased (χ2= 5.85, P < 0.05, p.c. = NS; χ2= 5.64, P < 0.05, p.c. = NS). There is no significant difference in frequency of DP alleles between male and female patients. Since a linkage disequilibrium between HLA‐B, DR and DP loci is found in our SLE patients, an analysis is performed assessing the relative importance of these HLA‐markers to SLE. The results show that the increase of DPB1*0101 in SLE patients is associated with the HLA‐B8, DR3 haplotype and it suggests a more important role for HLA‐B8, DR3 or genes within this haplotype than for DPB1*0101 in the genetic predisposition for SLE.

Original languageEnglish (US)
Pages (from-to)259-266
Number of pages8
JournalInternational Journal of Immunogenetics
Volume20
Issue number4
DOIs
StatePublished - Aug 1993

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ASJC Scopus subject areas

  • Immunology
  • Molecular Biology
  • Genetics
  • Genetics(clinical)

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