DNA supercoiling allows enhancer action over a large distance

Ye Liu, Vladimir Bondarenko, Alexander Ninfa, Vasily M. Studitsky

Research output: Contribution to journalArticle

Abstract

Enhancers are regulatory DNA elements that can activate their genomic targets over a large distance. The mechanism of enhancer action over large distance is unknown. Activation of the glnAp2 promoter by NtrC-dependent enhancer in Escherichia coli was analyzed by using a purified system supporting multiple-round transcription in vitro. The data suggest that DNA supercoiling is an essential requirement for enhancer action over a large distance (2,500 bp) but not over a short distance (110 bp). DNA supercoiling facilitates functional enhancer-promoter communication over a large distance, probably by bringing the enhancer and promoter into close proximity.

Original languageEnglish (US)
Pages (from-to)14883-14888
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume98
Issue number26
DOIs
StatePublished - Dec 18 2001

Keywords

  • Enhancers
  • Escherichia coli
  • NtrC activator
  • Transcription in vitro
  • glnAp2 gene

ASJC Scopus subject areas

  • General

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