DNA Strand breakage in chinese hamster v79 cells caused by low levels of incorporated [3h] and [14c]thymidine

Timothy J. Jorgensen, Peggy L. Olive, Ralph E. Durand

Research output: Contribution to journalArticle

Abstract

A sensitive alkali-unwinding assay was used to measure DNA strand breakage in Chinese hamster V79 cells caused by low-level incorporation of methyl-labelled [3H] and [14C] thymidine, and to estimate the effective dose per disintegration relative to low doses of gamma-irradiation. Damage equivalent to 0·0035 ± 0·0006 and 0·0014 ± 0·0005 Gy was observed for each 3H and 14C disintegration respectively. These values agree well with those expected from the estimated nuclear radiation dose delivered by the beta particles if a relative biological effect (r.b.e.) of 1·0 is assumed, and suggest that strand-breakage produced by these isotopes is determined by the nuclear radiation dose delivered by the beta particles.

Original languageEnglish (US)
Pages (from-to)673-680
Number of pages8
JournalInternational Journal of Radiation Biology
Volume51
Issue number4
DOIs
StatePublished - 1987

Fingerprint

Beta Particles
thymidine
hamsters
Chinese hamsters
Cricetulus
strands
Thymidine
Dosimetry
DNA
deoxyribonucleic acid
Disintegration
Radiation
nuclear radiation
dosage
beta particles
Alkalies
disintegration
cells
Isotopes
biological effects

Keywords

  • Beta particles
  • DNA damage
  • Low doses
  • Radiosotopes
  • Strand breaks

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology
  • Agricultural and Biological Sciences (miscellaneous)
  • Nuclear Energy and Engineering
  • Radiation

Cite this

DNA Strand breakage in chinese hamster v79 cells caused by low levels of incorporated [3h] and [14c]thymidine. / Jorgensen, Timothy J.; Olive, Peggy L.; Durand, Ralph E.

In: International Journal of Radiation Biology, Vol. 51, No. 4, 1987, p. 673-680.

Research output: Contribution to journalArticle

Jorgensen, Timothy J. ; Olive, Peggy L. ; Durand, Ralph E. / DNA Strand breakage in chinese hamster v79 cells caused by low levels of incorporated [3h] and [14c]thymidine. In: International Journal of Radiation Biology. 1987 ; Vol. 51, No. 4. pp. 673-680.
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