DNA sequence of a 3.8 kilobase pair region controlling Drosophila chorion gene amplification

Joseph Levine, Allan Spradling

Research output: Contribution to journalArticle

Abstract

During Drosophila oogenesis, two clusters of chorion genes and their flanking DNA sequences undergo amplification in the ovarian follicle cells. Amplification results from repeated rounds of initiation and bidirectional replication within the chorion gene regions, possibly from a single origin, producing nested replication forks. Previously we have shown that following reintroduction into the Drosophila genome, a specific 3.8 kilobase pair DNA segment from the amplified third chromosome domain could induce developmentally regulated amplification at its site of insertion. Here we present the complete nucleotide sequence of this "amplification control element" and of genes encoding the chorion structural proteins s18-1 and s15-1, which are contained within it. Sequences that may be involved in the regulation of chorion gene amplification and expression are identified.

Original languageEnglish (US)
Pages (from-to)136-142
Number of pages7
JournalChromosoma
Volume92
Issue number2
DOIs
StatePublished - Jun 1985
Externally publishedYes

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Chorion
Gene Amplification
Drosophila
Oogenesis
Ovarian Follicle
Multigene Family
Genes
Chromosomes
Genome
Gene Expression
DNA

ASJC Scopus subject areas

  • Genetics

Cite this

DNA sequence of a 3.8 kilobase pair region controlling Drosophila chorion gene amplification. / Levine, Joseph; Spradling, Allan.

In: Chromosoma, Vol. 92, No. 2, 06.1985, p. 136-142.

Research output: Contribution to journalArticle

Levine, Joseph ; Spradling, Allan. / DNA sequence of a 3.8 kilobase pair region controlling Drosophila chorion gene amplification. In: Chromosoma. 1985 ; Vol. 92, No. 2. pp. 136-142.
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