TY - JOUR
T1 - DNA segregation in Escherichia coli
T2 - Observations by means of tritiated thymidine decay
AU - Person, Stanley
AU - Osborn, Mary
PY - 1964
Y1 - 1964
N2 - Escherichia coli, strain 15T-L-, inactivation by H 3-thymidine decay was used in a study of DNA segregation. Two characteristics of the inactivation curves due to H3-thymidine decay, the initial slope and stable fraction, enable the mode of segregation to be defined. The results suggest that the segregation pattern is consistent with a semiconservative model of DNA replication. The data on the stable fraction indicate that the DNA of strain 15T-L-, under our experimental conditions, may be organized into one duplex structure. Since colony-forming ability was used as the means of assay, it is possible to compare these experiments with those in which DNA segregation is examined on a structural basis.
AB - Escherichia coli, strain 15T-L-, inactivation by H 3-thymidine decay was used in a study of DNA segregation. Two characteristics of the inactivation curves due to H3-thymidine decay, the initial slope and stable fraction, enable the mode of segregation to be defined. The results suggest that the segregation pattern is consistent with a semiconservative model of DNA replication. The data on the stable fraction indicate that the DNA of strain 15T-L-, under our experimental conditions, may be organized into one duplex structure. Since colony-forming ability was used as the means of assay, it is possible to compare these experiments with those in which DNA segregation is examined on a structural basis.
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U2 - 10.1126/science.143.3601.44
DO - 10.1126/science.143.3601.44
M3 - Article
C2 - 14075710
AN - SCOPUS:37049235112
SN - 0036-8075
VL - 143
SP - 44
EP - 46
JO - Science
JF - Science
IS - 3601
ER -