DNA restriction endonuclease analysis for localization of human β- and δ-globin genes on chromosome 11

A. F. Scott, J. A. Phillips, B. R. Migeon

Research output: Contribution to journalArticle

Abstract

DNA from a clone of a mouse-human hybrid that retained a human chromosome consisting of the major part of chromosome 11 and region q25-26-qter of the X chromosome was digested with various restriction endonucleases, subjected to electrophoresis in agarose gels, and transferred to nitrocellulose filters. The restriction digest pattern of the clone, when hybridized with a 32P-labeleld plasmid fragment containing human β-globin gene sequences, was a composite of the normal human and mouse (A9) patterns. When back-selected in 6-thioguanine to eliminate the 11 translocation chromosome, the hybrid cells showed only the A9 restriction pattern. These results substantiate the localization of β- and δ-globin genes to human chromosome 11 and exclude the region 11q23-qter as the site.

Original languageEnglish (US)
Pages (from-to)4563-4565
Number of pages3
JournalProceedings of the National Academy of Sciences of the United States of America
Volume76
Issue number9
DOIs
StatePublished - Jan 1 1979
Externally publishedYes

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ASJC Scopus subject areas

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