DNA prime Listeria boost induces a cellular immune response to SIV antigens in the rhesus macaque model that is capable of limited suppression of SIV239 viral replication

Jean D. Boyer, Tara M. Robinson, Paulo C. Maciag, Xiaohui Peng, Ross S. Johnson, George Pavlakis, Mark G. Lewis, Anding Shen, Robert F Siliciano, Charles R. Brown, David B. Weiner, Yvonne Paterson

Research output: Contribution to journalArticle

Abstract

DNA vaccines and recombinant Listeria monocytogenes that express and secrete SIV Gag and Env antigens were combined in a nonhuman primate prime-boost immunogenicity study followed by a challenge with SIV239. We report that recombinant DNA vaccine delivered intramuscularly, and recombinant L. monocytogenes delivered orally each individually have the ability to induce CD8+ and CD4+ T cell immune responses in a nonhuman primate. Four rhesus monkeys were immunized at weeks 0, 4, 8, and 12 with the pCSIVgag and pCSIVenv DNA plasmids and boosted with SIV expressing L. monocytogenes vaccines at weeks 16, 20, and 28. Four rhesus monkeys received only the L. monocytogenes vaccines at weeks 16, 20, and 28. A final group of monkeys served as a control group. Blood samples were taken before vaccination and 2 weeks post each injection and analyzed by ELISPOT for CD4+ and CD8+ T cell responses. Moderate vaccine induced SIV-specific cellular immune responses were observed following immunization with either DNA or L. monocytogenes vectors. However, the SIV antigen-specific immune responses were significantly increased when Rhesus macaques were primed with SIV DNA vaccines and boosted with the SIV expressing L. monocytogenes vectors. In addition, the combined vaccine was able to impact SIV239 viral replication following an intrarectal challenge. This study demonstrates for the first time that oral L. monocytogenes can induce a cellular immune response in a nonhuman primate and is able to enhance the efficacy of a DNA vaccine as well as provide modest protection against SIV239 challenge.

Original languageEnglish (US)
Pages (from-to)88-101
Number of pages14
JournalVirology
Volume333
Issue number1
DOIs
StatePublished - Mar 1 2005

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Listeria
Listeria monocytogenes
Macaca mulatta
Cellular Immunity
DNA Vaccines
Antigens
DNA
SAIDS Vaccines
Primates
Vaccines
env Gene Products
Combined Vaccines
T-Lymphocytes
gag Gene Products
Enzyme-Linked Immunospot Assay
Histocompatibility Antigens Class II
Haplorhini
Immunization
Vaccination
Plasmids

Keywords

  • DNA vaccine
  • HIV/SIV vaccine
  • Listeria monocytogenes
  • Prime/boost

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

DNA prime Listeria boost induces a cellular immune response to SIV antigens in the rhesus macaque model that is capable of limited suppression of SIV239 viral replication. / Boyer, Jean D.; Robinson, Tara M.; Maciag, Paulo C.; Peng, Xiaohui; Johnson, Ross S.; Pavlakis, George; Lewis, Mark G.; Shen, Anding; Siliciano, Robert F; Brown, Charles R.; Weiner, David B.; Paterson, Yvonne.

In: Virology, Vol. 333, No. 1, 01.03.2005, p. 88-101.

Research output: Contribution to journalArticle

Boyer, JD, Robinson, TM, Maciag, PC, Peng, X, Johnson, RS, Pavlakis, G, Lewis, MG, Shen, A, Siliciano, RF, Brown, CR, Weiner, DB & Paterson, Y 2005, 'DNA prime Listeria boost induces a cellular immune response to SIV antigens in the rhesus macaque model that is capable of limited suppression of SIV239 viral replication', Virology, vol. 333, no. 1, pp. 88-101. https://doi.org/10.1016/j.virol.2004.12.026
Boyer, Jean D. ; Robinson, Tara M. ; Maciag, Paulo C. ; Peng, Xiaohui ; Johnson, Ross S. ; Pavlakis, George ; Lewis, Mark G. ; Shen, Anding ; Siliciano, Robert F ; Brown, Charles R. ; Weiner, David B. ; Paterson, Yvonne. / DNA prime Listeria boost induces a cellular immune response to SIV antigens in the rhesus macaque model that is capable of limited suppression of SIV239 viral replication. In: Virology. 2005 ; Vol. 333, No. 1. pp. 88-101.
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abstract = "DNA vaccines and recombinant Listeria monocytogenes that express and secrete SIV Gag and Env antigens were combined in a nonhuman primate prime-boost immunogenicity study followed by a challenge with SIV239. We report that recombinant DNA vaccine delivered intramuscularly, and recombinant L. monocytogenes delivered orally each individually have the ability to induce CD8+ and CD4+ T cell immune responses in a nonhuman primate. Four rhesus monkeys were immunized at weeks 0, 4, 8, and 12 with the pCSIVgag and pCSIVenv DNA plasmids and boosted with SIV expressing L. monocytogenes vaccines at weeks 16, 20, and 28. Four rhesus monkeys received only the L. monocytogenes vaccines at weeks 16, 20, and 28. A final group of monkeys served as a control group. Blood samples were taken before vaccination and 2 weeks post each injection and analyzed by ELISPOT for CD4+ and CD8+ T cell responses. Moderate vaccine induced SIV-specific cellular immune responses were observed following immunization with either DNA or L. monocytogenes vectors. However, the SIV antigen-specific immune responses were significantly increased when Rhesus macaques were primed with SIV DNA vaccines and boosted with the SIV expressing L. monocytogenes vectors. In addition, the combined vaccine was able to impact SIV239 viral replication following an intrarectal challenge. This study demonstrates for the first time that oral L. monocytogenes can induce a cellular immune response in a nonhuman primate and is able to enhance the efficacy of a DNA vaccine as well as provide modest protection against SIV239 challenge.",
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