Abstract
DNA vaccines and recombinant Listeria monocytogenes that express and secrete SIV Gag and Env antigens were combined in a nonhuman primate prime-boost immunogenicity study followed by a challenge with SIV239. We report that recombinant DNA vaccine delivered intramuscularly, and recombinant L. monocytogenes delivered orally each individually have the ability to induce CD8+ and CD4+ T cell immune responses in a nonhuman primate. Four rhesus monkeys were immunized at weeks 0, 4, 8, and 12 with the pCSIVgag and pCSIVenv DNA plasmids and boosted with SIV expressing L. monocytogenes vaccines at weeks 16, 20, and 28. Four rhesus monkeys received only the L. monocytogenes vaccines at weeks 16, 20, and 28. A final group of monkeys served as a control group. Blood samples were taken before vaccination and 2 weeks post each injection and analyzed by ELISPOT for CD4+ and CD8+ T cell responses. Moderate vaccine induced SIV-specific cellular immune responses were observed following immunization with either DNA or L. monocytogenes vectors. However, the SIV antigen-specific immune responses were significantly increased when Rhesus macaques were primed with SIV DNA vaccines and boosted with the SIV expressing L. monocytogenes vectors. In addition, the combined vaccine was able to impact SIV239 viral replication following an intrarectal challenge. This study demonstrates for the first time that oral L. monocytogenes can induce a cellular immune response in a nonhuman primate and is able to enhance the efficacy of a DNA vaccine as well as provide modest protection against SIV239 challenge.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 88-101 |
| Number of pages | 14 |
| Journal | Virology |
| Volume | 333 |
| Issue number | 1 |
| DOIs | |
| State | Published - Mar 1 2005 |
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Keywords
- DNA vaccine
- HIV/SIV vaccine
- Listeria monocytogenes
- Prime/boost
ASJC Scopus subject areas
- Virology
- Infectious Diseases
Cite this
DNA prime Listeria boost induces a cellular immune response to SIV antigens in the rhesus macaque model that is capable of limited suppression of SIV239 viral replication. / Boyer, Jean D.; Robinson, Tara M.; Maciag, Paulo C.; Peng, Xiaohui; Johnson, Ross S.; Pavlakis, George; Lewis, Mark G.; Shen, Anding; Siliciano, Robert F; Brown, Charles R.; Weiner, David B.; Paterson, Yvonne.
In: Virology, Vol. 333, No. 1, 01.03.2005, p. 88-101.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - DNA prime Listeria boost induces a cellular immune response to SIV antigens in the rhesus macaque model that is capable of limited suppression of SIV239 viral replication
AU - Boyer, Jean D.
AU - Robinson, Tara M.
AU - Maciag, Paulo C.
AU - Peng, Xiaohui
AU - Johnson, Ross S.
AU - Pavlakis, George
AU - Lewis, Mark G.
AU - Shen, Anding
AU - Siliciano, Robert F
AU - Brown, Charles R.
AU - Weiner, David B.
AU - Paterson, Yvonne
PY - 2005/3/1
Y1 - 2005/3/1
N2 - DNA vaccines and recombinant Listeria monocytogenes that express and secrete SIV Gag and Env antigens were combined in a nonhuman primate prime-boost immunogenicity study followed by a challenge with SIV239. We report that recombinant DNA vaccine delivered intramuscularly, and recombinant L. monocytogenes delivered orally each individually have the ability to induce CD8+ and CD4+ T cell immune responses in a nonhuman primate. Four rhesus monkeys were immunized at weeks 0, 4, 8, and 12 with the pCSIVgag and pCSIVenv DNA plasmids and boosted with SIV expressing L. monocytogenes vaccines at weeks 16, 20, and 28. Four rhesus monkeys received only the L. monocytogenes vaccines at weeks 16, 20, and 28. A final group of monkeys served as a control group. Blood samples were taken before vaccination and 2 weeks post each injection and analyzed by ELISPOT for CD4+ and CD8+ T cell responses. Moderate vaccine induced SIV-specific cellular immune responses were observed following immunization with either DNA or L. monocytogenes vectors. However, the SIV antigen-specific immune responses were significantly increased when Rhesus macaques were primed with SIV DNA vaccines and boosted with the SIV expressing L. monocytogenes vectors. In addition, the combined vaccine was able to impact SIV239 viral replication following an intrarectal challenge. This study demonstrates for the first time that oral L. monocytogenes can induce a cellular immune response in a nonhuman primate and is able to enhance the efficacy of a DNA vaccine as well as provide modest protection against SIV239 challenge.
AB - DNA vaccines and recombinant Listeria monocytogenes that express and secrete SIV Gag and Env antigens were combined in a nonhuman primate prime-boost immunogenicity study followed by a challenge with SIV239. We report that recombinant DNA vaccine delivered intramuscularly, and recombinant L. monocytogenes delivered orally each individually have the ability to induce CD8+ and CD4+ T cell immune responses in a nonhuman primate. Four rhesus monkeys were immunized at weeks 0, 4, 8, and 12 with the pCSIVgag and pCSIVenv DNA plasmids and boosted with SIV expressing L. monocytogenes vaccines at weeks 16, 20, and 28. Four rhesus monkeys received only the L. monocytogenes vaccines at weeks 16, 20, and 28. A final group of monkeys served as a control group. Blood samples were taken before vaccination and 2 weeks post each injection and analyzed by ELISPOT for CD4+ and CD8+ T cell responses. Moderate vaccine induced SIV-specific cellular immune responses were observed following immunization with either DNA or L. monocytogenes vectors. However, the SIV antigen-specific immune responses were significantly increased when Rhesus macaques were primed with SIV DNA vaccines and boosted with the SIV expressing L. monocytogenes vectors. In addition, the combined vaccine was able to impact SIV239 viral replication following an intrarectal challenge. This study demonstrates for the first time that oral L. monocytogenes can induce a cellular immune response in a nonhuman primate and is able to enhance the efficacy of a DNA vaccine as well as provide modest protection against SIV239 challenge.
KW - DNA vaccine
KW - HIV/SIV vaccine
KW - Listeria monocytogenes
KW - Prime/boost
UR - http://www.scopus.com/inward/record.url?scp=13544261553&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=13544261553&partnerID=8YFLogxK
U2 - 10.1016/j.virol.2004.12.026
DO - 10.1016/j.virol.2004.12.026
M3 - Article
C2 - 15708595
AN - SCOPUS:13544261553
VL - 333
SP - 88
EP - 101
JO - Virology
JF - Virology
SN - 0042-6822
IS - 1
ER -