DNA methylation regulates microRNA expression

Liangfeng Han, P. Dane Witmer, Emily Casey, David Valle, Saraswati Sukumar

Research output: Contribution to journalArticle

Abstract

MicroRNAs (miRNAs), an important class of small regulatory molecules for gene expression, are transcribed by RNA polymerase II. But little is known about the mechanisms that control miRNA expression. Comparing miRNA expression profiles between colon cancer cell line HCT 116 and its derivative, DNA methyltransferase 1 and 3b (DNMT1 and DNMT3b) double knockout cell line, we found that the expression of about 10% miRNAs was regulated by DNA methylation. In addition, neither 5-aza-2′-deoxycytidine treatment nor deletion of DNMT1 alone recapitulated miRNA expression profile seen in the double knockout cell line, suggesting that miRNA expression was tightly controlled by DNA methylation and partial methylation reduction was not sufficient for miRNA reexpression. We also found that HOXA3 and HOXD10 were putative targets of mir-10a, one of the differentially expressed miRNAs that is located in HOX gene cluster.

Original languageEnglish (US)
Pages (from-to)1290-1294
Number of pages5
JournalCancer Biology and Therapy
Volume6
Issue number8
StatePublished - Aug 2007

    Fingerprint

Keywords

  • DNA methylation
  • Epigenetics
  • Expression regulation
  • HOXA3
  • HOXD10
  • Knockout
  • MicroRNA
  • MicroRNA array
  • QRT-PCR

ASJC Scopus subject areas

  • Molecular Medicine
  • Oncology
  • Pharmacology
  • Cancer Research

Cite this