DNA methylation analysis on a droplet-in-oil PCR array

Yi Zhang, Vasudev Bailey, Christopher M. Puleo, Hariharan Easwaran, Elizabeth Griffiths, James G. Herman, Stephen B Baylin, Tza Huei Wang

Research output: Contribution to journalArticle

Abstract

We performed on-chip DNA methylation analysis using methylation-specific PCR (MSP) within an arrayed micro droplet-in-oil platform that is designed for more practical application of microfluidic droplet technologies in clinical applications. Unique features of this ready-to-use device include arrayed primers that are pre-deposited into open micro-reaction chambers and use of the oil phase as a companion fluid for both sample actuation and compartmentalization. These technical advantages allow for infusion of minute amounts of sample for arrayed MSP analysis, without the added complexities inherent in microfluidic droplet-based studies. Ease of use of this micro device is exemplified by analysis of two tumor suppressor promoters, p15 and TMS1 using an on-chip methylation assay. These results were consistent with standard MSP protocols, yet the simplicity of the droplet-in-oil microfluidic PCR platform provides an easy and efficient tool for DNA methylation analysis in a large-scale arrayed manner.

Original languageEnglish (US)
Pages (from-to)1059-1064
Number of pages6
JournalLab on a Chip - Miniaturisation for Chemistry and Biology
Volume9
Issue number8
DOIs
StatePublished - 2009

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Methylation
DNA Methylation
Microfluidics
Oils
Polymerase Chain Reaction
Equipment and Supplies
Carcinogens
Tumors
Assays
Technology
Fluids

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)
  • Bioengineering
  • Biomedical Engineering

Cite this

Zhang, Y., Bailey, V., Puleo, C. M., Easwaran, H., Griffiths, E., Herman, J. G., ... Wang, T. H. (2009). DNA methylation analysis on a droplet-in-oil PCR array. Lab on a Chip - Miniaturisation for Chemistry and Biology, 9(8), 1059-1064. https://doi.org/10.1039/b821780g

DNA methylation analysis on a droplet-in-oil PCR array. / Zhang, Yi; Bailey, Vasudev; Puleo, Christopher M.; Easwaran, Hariharan; Griffiths, Elizabeth; Herman, James G.; Baylin, Stephen B; Wang, Tza Huei.

In: Lab on a Chip - Miniaturisation for Chemistry and Biology, Vol. 9, No. 8, 2009, p. 1059-1064.

Research output: Contribution to journalArticle

Zhang, Yi ; Bailey, Vasudev ; Puleo, Christopher M. ; Easwaran, Hariharan ; Griffiths, Elizabeth ; Herman, James G. ; Baylin, Stephen B ; Wang, Tza Huei. / DNA methylation analysis on a droplet-in-oil PCR array. In: Lab on a Chip - Miniaturisation for Chemistry and Biology. 2009 ; Vol. 9, No. 8. pp. 1059-1064.
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