DNA methylation alterations exhibit intraindividual stability and interindividual heterogeneity in prostate cancer metastases

Martin J. Aryee, Wennuan Liu, Julia C. Engelmann, Philipp Nuhn, Meltem Gurel, Michael C. Haffner, David Esopi, Rafael A. Irizarry, Robert H. Getzenberg, William G Nelson, Jun Luo, Jianfeng Xu, William B Isaacs, G. Steven Bova, S Yegnasubramanian

Research output: Contribution to journalArticle

Abstract

Human cancers almost ubiquitously harbor epigenetic alterations. Although such alterations in epigenetic marks, including DNA methylation, are potentially heritable, they can also be dynamically altered. Given this potential for plasticity, the degree to which epigenetic changes can be subject to selection and act as drivers of neoplasia has been questioned. We carried out genome-scale analyses of DNA methylation alterations in lethal metastatic prostate cancer and created DNA methylation "cityscape" plots to visualize these complex data. We show that somatic DNA methylation alterations, despite showing marked interindividual heterogeneity among men with lethal metastatic prostate cancer, were maintained across all metastases within the same individual. The overall extent of maintenance in DNA methylation changes was comparable to that of genetic copy number alterations. Regions that were frequently hypermethylated across individuals were markedly enriched for cancer- and development/ differentiation-related genes. Additionally, regions exhibiting high consistency of hypermethylation across metastases within individuals, even if variably hypermethylated across individuals, showed enrichment for cancer-related genes. Whereas some regions showed intraindividual metastatic tumor heterogeneity in promoter methylation, such methylation alterations were generally not correlated with gene expression. This was despite a general tendency for promoter methylation patterns to be strongly correlated with gene expression, particularly at regions that were variably methylated across individuals. These findings suggest that DNA methylation alterations have the potential for producing selectable driver events in carcinogenesis and disease progression and highlight the possibility of targeting such epigenome alterations for development of longitudinal markers and therapeutic strategies.

Original languageEnglish (US)
Article number169ra10
JournalScience Translational Medicine
Volume5
Issue number169
DOIs
StatePublished - Jan 23 2013

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DNA Methylation
Prostatic Neoplasms
Neoplasm Metastasis
Epigenomics
Methylation
Neoplasms
Gene Expression
Neoplasm Genes
Patient Selection
Disease Progression
Carcinogenesis
Maintenance
Genome
Genes

ASJC Scopus subject areas

  • Medicine(all)

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DNA methylation alterations exhibit intraindividual stability and interindividual heterogeneity in prostate cancer metastases. / Aryee, Martin J.; Liu, Wennuan; Engelmann, Julia C.; Nuhn, Philipp; Gurel, Meltem; Haffner, Michael C.; Esopi, David; Irizarry, Rafael A.; Getzenberg, Robert H.; Nelson, William G; Luo, Jun; Xu, Jianfeng; Isaacs, William B; Bova, G. Steven; Yegnasubramanian, S.

In: Science Translational Medicine, Vol. 5, No. 169, 169ra10, 23.01.2013.

Research output: Contribution to journalArticle

Aryee, Martin J. ; Liu, Wennuan ; Engelmann, Julia C. ; Nuhn, Philipp ; Gurel, Meltem ; Haffner, Michael C. ; Esopi, David ; Irizarry, Rafael A. ; Getzenberg, Robert H. ; Nelson, William G ; Luo, Jun ; Xu, Jianfeng ; Isaacs, William B ; Bova, G. Steven ; Yegnasubramanian, S. / DNA methylation alterations exhibit intraindividual stability and interindividual heterogeneity in prostate cancer metastases. In: Science Translational Medicine. 2013 ; Vol. 5, No. 169.
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AU - Gurel, Meltem

AU - Haffner, Michael C.

AU - Esopi, David

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AU - Luo, Jun

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