TY - JOUR
T1 - DNA mechanics and its biological impact
AU - Basu, Aakash
AU - Bobrovnikov, Dmitriy G.
AU - Ha, Taekjip
N1 - Funding Information:
This work was supported by the National Science Foundation Grants PHY-1430124, EFMA 1933303 (to T.H.), and the National Institutes of Health Grants GM122569 (to T.H.), T.H. is an investigator with the Howard Hughes Medical Institute.
Publisher Copyright:
© 2021 Elsevier Ltd
PY - 2021/3/19
Y1 - 2021/3/19
N2 - Almost all nucleoprotein interactions and DNA manipulation events involve mechanical deformations of DNA. Extraordinary progresses in single-molecule, structural, and computational methods have characterized the average mechanical properties of DNA, such as bendability and torsional rigidity, in high resolution. Further, the advent of sequencing technology has permitted measuring, in high-throughput, how such mechanical properties vary with sequence and epigenetic modifications along genomes. We review these recent technological advancements, and discuss how they have contributed to the emerging idea that variations in the mechanical properties of DNA play a fundamental role in regulating, genome-wide, diverse processes involved in chromatin organization.
AB - Almost all nucleoprotein interactions and DNA manipulation events involve mechanical deformations of DNA. Extraordinary progresses in single-molecule, structural, and computational methods have characterized the average mechanical properties of DNA, such as bendability and torsional rigidity, in high resolution. Further, the advent of sequencing technology has permitted measuring, in high-throughput, how such mechanical properties vary with sequence and epigenetic modifications along genomes. We review these recent technological advancements, and discuss how they have contributed to the emerging idea that variations in the mechanical properties of DNA play a fundamental role in regulating, genome-wide, diverse processes involved in chromatin organization.
KW - DNA physics
KW - chromatin biophysics
KW - genomics
KW - mechanobiology of DNA
KW - singlemolecule biophysics
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U2 - 10.1016/j.jmb.2021.166861
DO - 10.1016/j.jmb.2021.166861
M3 - Review article
C2 - 33539885
AN - SCOPUS:85101168015
SN - 0022-2836
VL - 433
JO - Journal of Molecular Biology
JF - Journal of Molecular Biology
IS - 6
M1 - 166861
ER -