DNA image cytometry in MDS bone marrow smears

Susanne Widell, Robert Hast, Gert Auer, Christopher Cox, John M. Bennett

Research output: Contribution to journalArticlepeer-review

Abstract

The nuclear DNA content, defined as DNA index (DI) in blasts/promyelocytes (bla/pro), were determined on Feulgen-stained bone marrow smears from 39 patients with myelodysplastic syndromes (MDS) and eight control subjects by the use of image cytometry (ICM). The DI in patients was compared to that of corresponding normal cell types, and to cytogenetic data available in 32/39 patients. The mean DI in bla/pro of patients with MDS was significantly (P<0.01) lower compared to corresponding cell types in control subjects. By ICM, a DNA aneuploidy in bla/pro was found in 67% of the MDS patients, and 59% expressed DNA hypodiploidy. By cytogenetics, an abnormal karyotype was found in 31%, and 6/9 MDS patients with a 'hypodiploid' abnormal karyotype showed DNA hypodiploid. Of patients with a normal karyotype (69%), seven (32%) showed a normal, 12 (55%) a lower, and three (14%) a higher DI compared to controls. No difference between groups of MDS patients was found. DNA hypodiploidy is suggested to be a common feature in MDS without a relationship to cytogenetics.

Original languageEnglish (US)
Pages (from-to)960-965
Number of pages6
JournalBritish journal of haematology
Volume105
Issue number4
DOIs
StatePublished - 1999
Externally publishedYes

Keywords

  • Cytogenetics
  • DNA cytometry
  • Intramarrow cell death
  • Leukaemia
  • MDS

ASJC Scopus subject areas

  • Hematology

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