DNA content measurements and an improved idiogram for the Indian muntjac

Howard P. Levy, Roger A. Schultz, José V. Ordonez, Maimon M. Cohen

Research output: Contribution to journalArticlepeer-review

Abstract

The Indian muntjac, an asiatic deer, has the lowest diploid chromosome number among mammals (female 2N = 6; male 2N = 7). Using flow cytometric quantification of propidium iodide‐stained cells, we determined the DNA content of muntjac cells to be 94% that of human. This suggests that the muntjac may serve as a model for investigation of karyotypic evolution and rearrangement. In order to facilitate future comparative gene mapping studies, computer‐aided analysis of digitized metaphase chromosomes allowed development of a detailed Indian muntjac G‐banded idiogram incorporating both ISCN‐type nomenclature and quantitative estimates of the size of each band and position. © 1993 Wiley‐Liss, Inc.

Original languageEnglish (US)
Pages (from-to)362-368
Number of pages7
JournalCytometry
Volume14
Issue number4
DOIs
StatePublished - 1993
Externally publishedYes

Keywords

  • Cellular DNA content
  • G‐band idiogram
  • image analysis

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Biophysics
  • Hematology
  • Endocrinology
  • Cell Biology

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