DNA cloning of plasmodium falciparum circumsporozoite gene: amino acid sequence of repetitive epitope

Vincenzo Enea, Joan Ellis, Fidel Zavala, David E. Arnot, Achara Asavanich, Aoi Masuda, Isabella Quakyi, Ruth S. Nussenzweig

Research output: Contribution to journalArticlepeer-review

Abstract

A clone of complementary DNA encoding the circumsporozoite (CS) protein of the human malaria parasite Plasmodium falciparum has been isolated by screening an Escherichia coli complementary DNA library with a monoclonal antibody to the CS protein. The DNA sequence of the complementary DNA insert encodes a four-amino acid sequence: proline-asparagine-alanine-asparagine, tandemly repeated 23 times. The CS β-lactamase fusion protein specifically binds monoclonal antibodies to the CS protein and inhibits the binding of these antibodies to native Plasmodium falciparum CS protein. These findings provide a basis for the development of a vaccine against Plasmodium falciparum malaria.

Original languageEnglish (US)
Pages (from-to)628-630
Number of pages3
JournalScience
Volume225
Issue number4662
DOIs
StatePublished - 1984
Externally publishedYes

ASJC Scopus subject areas

  • General

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