DNA cloning of plasmodium falciparum circumsporozoite gene: amino acid sequence of repetitive epitope

Vincenzo Enea; Joan Ellis; Fidel Zavala; David E. Arnot; Achara Asavanich; Aoi Masuda; Isabella Quakyi; Ruth S. Nussenzweig

doi:10.1126/science.6204384

DNA cloning of plasmodium falciparum circumsporozoite gene: amino acid sequence of repetitive epitope

Vincenzo Enea, Joan Ellis, Fidel Zavala, David E. Arnot, Achara Asavanich, Aoi Masuda, Isabella Quakyi, Ruth S. Nussenzweig

Research output: Contribution to journal › Article › peer-review

161 Scopus citations

Abstract

A clone of complementary DNA encoding the circumsporozoite (CS) protein of the human malaria parasite Plasmodium falciparum has been isolated by screening an Escherichia coli complementary DNA library with a monoclonal antibody to the CS protein. The DNA sequence of the complementary DNA insert encodes a four-amino acid sequence: proline-asparagine-alanine-asparagine, tandemly repeated 23 times. The CS β-lactamase fusion protein specifically binds monoclonal antibodies to the CS protein and inhibits the binding of these antibodies to native Plasmodium falciparum CS protein. These findings provide a basis for the development of a vaccine against Plasmodium falciparum malaria.

Original language	English (US)
Pages (from-to)	628-630
Number of pages	3
Journal	Science
Volume	225
Issue number	4662
DOIs	https://doi.org/10.1126/science.6204384
State	Published - 1984
Externally published	Yes

ASJC Scopus subject areas

General

Access to Document

10.1126/science.6204384

Cite this

@article{48998d3c4f164c8dad095096f4dbfc7c,

title = "DNA cloning of plasmodium falciparum circumsporozoite gene: amino acid sequence of repetitive epitope",

abstract = "A clone of complementary DNA encoding the circumsporozoite (CS) protein of the human malaria parasite Plasmodium falciparum has been isolated by screening an Escherichia coli complementary DNA library with a monoclonal antibody to the CS protein. The DNA sequence of the complementary DNA insert encodes a four-amino acid sequence: proline-asparagine-alanine-asparagine, tandemly repeated 23 times. The CS β-lactamase fusion protein specifically binds monoclonal antibodies to the CS protein and inhibits the binding of these antibodies to native Plasmodium falciparum CS protein. These findings provide a basis for the development of a vaccine against Plasmodium falciparum malaria.",

author = "Vincenzo Enea and Joan Ellis and Fidel Zavala and Arnot, {David E.} and Achara Asavanich and Aoi Masuda and Isabella Quakyi and Nussenzweig, {Ruth S.}",

year = "1984",

doi = "10.1126/science.6204384",

language = "English (US)",

volume = "225",

pages = "628--630",

journal = "Science",

issn = "0036-8075",

publisher = "American Association for the Advancement of Science",

number = "4662",

}

TY - JOUR

T1 - DNA cloning of plasmodium falciparum circumsporozoite gene

T2 - amino acid sequence of repetitive epitope

AU - Enea, Vincenzo

AU - Ellis, Joan

AU - Zavala, Fidel

AU - Arnot, David E.

AU - Asavanich, Achara

AU - Masuda, Aoi

AU - Quakyi, Isabella

AU - Nussenzweig, Ruth S.

PY - 1984

Y1 - 1984

N2 - A clone of complementary DNA encoding the circumsporozoite (CS) protein of the human malaria parasite Plasmodium falciparum has been isolated by screening an Escherichia coli complementary DNA library with a monoclonal antibody to the CS protein. The DNA sequence of the complementary DNA insert encodes a four-amino acid sequence: proline-asparagine-alanine-asparagine, tandemly repeated 23 times. The CS β-lactamase fusion protein specifically binds monoclonal antibodies to the CS protein and inhibits the binding of these antibodies to native Plasmodium falciparum CS protein. These findings provide a basis for the development of a vaccine against Plasmodium falciparum malaria.

AB - A clone of complementary DNA encoding the circumsporozoite (CS) protein of the human malaria parasite Plasmodium falciparum has been isolated by screening an Escherichia coli complementary DNA library with a monoclonal antibody to the CS protein. The DNA sequence of the complementary DNA insert encodes a four-amino acid sequence: proline-asparagine-alanine-asparagine, tandemly repeated 23 times. The CS β-lactamase fusion protein specifically binds monoclonal antibodies to the CS protein and inhibits the binding of these antibodies to native Plasmodium falciparum CS protein. These findings provide a basis for the development of a vaccine against Plasmodium falciparum malaria.

UR - http://www.scopus.com/inward/record.url?scp=0021160478&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021160478&partnerID=8YFLogxK

U2 - 10.1126/science.6204384

DO - 10.1126/science.6204384

M3 - Article

C2 - 6204384

AN - SCOPUS:0021160478

SN - 0036-8075

VL - 225

SP - 628

EP - 630

JO - Science

JF - Science

IS - 4662

ER -

DNA cloning of plasmodium falciparum circumsporozoite gene: amino acid sequence of repetitive epitope

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this