DNA cloning of plasmodium falciparum circumsporozoite gene: amino acid sequence of repetitive epitope

Vincenzo Enea, Joan Ellis, Fidel Zavala, David E. Arnot, Achara Asavanich, Aoi Masuda, Isabella Quakyi, Ruth S. Nussenzweig

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Abstract

A clone of complementary DNA encoding the circumsporozoite (CS) protein of the human malaria parasite Plasmodium falciparum has been isolated by screening an Escherichia coli complementary DNA library with a monoclonal antibody to the CS protein. The DNA sequence of the complementary DNA insert encodes a four-amino acid sequence: proline-asparagine-alanine-asparagine, tandemly repeated 23 times. The CS β-lactamase fusion protein specifically binds monoclonal antibodies to the CS protein and inhibits the binding of these antibodies to native Plasmodium falciparum CS protein. These findings provide a basis for the development of a vaccine against Plasmodium falciparum malaria.

Original languageEnglish (US)
Pages (from-to)628-630
Number of pages3
JournalScience
Volume225
Issue number4662
DOIs
StatePublished - Jan 1 1984

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Cite this

Enea, V., Ellis, J., Zavala, F., Arnot, D. E., Asavanich, A., Masuda, A., Quakyi, I., & Nussenzweig, R. S. (1984). DNA cloning of plasmodium falciparum circumsporozoite gene: amino acid sequence of repetitive epitope. Science, 225(4662), 628-630. https://doi.org/10.1126/science.6204384