DNA and RNA damage by Cu(II)-amikacin complex

Malgorzata Jezowska-Bojczuk; Wojciech Szczepanik; Wojciech Lesniak; Jerzy Ciesiolka; Jan Wrzesinski; Wojciech Bal

doi:10.1046/j.1432-1033.2002.03260.x

DNA and RNA damage by Cu(II)-amikacin complex

Malgorzata Jezowska-Bojczuk, Wojciech Szczepanik, Wojciech Lesniak, Jerzy Ciesiolka, Jan Wrzesinski, Wojciech Bal

Research output: Contribution to journal › Article › peer-review

32 Scopus citations

Abstract

The oxidation-promoting reactivity of copper(II) complex of aminoglycosidic antibiotic amikacin [Cu(II)-Ami] in the presence of hydrogen peroxide, was studied at pH 7.4, using 2′-deoxyguanosine (dG), pBR322 plasmid DNA and yeast tRNA^Phe as target molecules. The mixtures of complex with H₂O₂ were found to be efficient oxidants, converting dG to its 8-oxo derivative, generating strand breaks in plasmid DNA and multiple cleavages in tRNA^Phe. The complex underwent autooxidation as well, with amikacin hydroperoxides as likely major products. This reactivity pattern was found to be due to a combination of metal-bound and free hydroxyl radicals.

Original language	English (US)
Pages (from-to)	5547-5556
Number of pages	10
Journal	European Journal of Biochemistry
Volume	269
Issue number	22
DOIs	https://doi.org/10.1046/j.1432-1033.2002.03260.x
State	Published - 2002
Externally published	Yes

Keywords

2′-deoxyguanosine oxidation
Amikacin
Copper(II) complexes
Plasmid DNA damage
tRNA cleavage

ASJC Scopus subject areas

Biochemistry

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10.1046/j.1432-1033.2002.03260.x

Cite this

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title = "DNA and RNA damage by Cu(II)-amikacin complex",

abstract = "The oxidation-promoting reactivity of copper(II) complex of aminoglycosidic antibiotic amikacin [Cu(II)-Ami] in the presence of hydrogen peroxide, was studied at pH 7.4, using 2′-deoxyguanosine (dG), pBR322 plasmid DNA and yeast tRNAPhe as target molecules. The mixtures of complex with H2O2 were found to be efficient oxidants, converting dG to its 8-oxo derivative, generating strand breaks in plasmid DNA and multiple cleavages in tRNAPhe. The complex underwent autooxidation as well, with amikacin hydroperoxides as likely major products. This reactivity pattern was found to be due to a combination of metal-bound and free hydroxyl radicals.",

keywords = "2′-deoxyguanosine oxidation, Amikacin, Copper(II) complexes, Plasmid DNA damage, tRNA cleavage",

author = "Malgorzata Jezowska-Bojczuk and Wojciech Szczepanik and Wojciech Lesniak and Jerzy Ciesiolka and Jan Wrzesinski and Wojciech Bal",

year = "2002",

doi = "10.1046/j.1432-1033.2002.03260.x",

language = "English (US)",

volume = "269",

pages = "5547--5556",

journal = "European Journal of Biochemistry",

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publisher = "Wiley-Blackwell",

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TY - JOUR

T1 - DNA and RNA damage by Cu(II)-amikacin complex

AU - Jezowska-Bojczuk, Malgorzata

AU - Szczepanik, Wojciech

AU - Lesniak, Wojciech

AU - Ciesiolka, Jerzy

AU - Wrzesinski, Jan

AU - Bal, Wojciech

PY - 2002

Y1 - 2002

N2 - The oxidation-promoting reactivity of copper(II) complex of aminoglycosidic antibiotic amikacin [Cu(II)-Ami] in the presence of hydrogen peroxide, was studied at pH 7.4, using 2′-deoxyguanosine (dG), pBR322 plasmid DNA and yeast tRNAPhe as target molecules. The mixtures of complex with H2O2 were found to be efficient oxidants, converting dG to its 8-oxo derivative, generating strand breaks in plasmid DNA and multiple cleavages in tRNAPhe. The complex underwent autooxidation as well, with amikacin hydroperoxides as likely major products. This reactivity pattern was found to be due to a combination of metal-bound and free hydroxyl radicals.

AB - The oxidation-promoting reactivity of copper(II) complex of aminoglycosidic antibiotic amikacin [Cu(II)-Ami] in the presence of hydrogen peroxide, was studied at pH 7.4, using 2′-deoxyguanosine (dG), pBR322 plasmid DNA and yeast tRNAPhe as target molecules. The mixtures of complex with H2O2 were found to be efficient oxidants, converting dG to its 8-oxo derivative, generating strand breaks in plasmid DNA and multiple cleavages in tRNAPhe. The complex underwent autooxidation as well, with amikacin hydroperoxides as likely major products. This reactivity pattern was found to be due to a combination of metal-bound and free hydroxyl radicals.

KW - 2′-deoxyguanosine oxidation

KW - Amikacin

KW - Copper(II) complexes

KW - Plasmid DNA damage

KW - tRNA cleavage

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DO - 10.1046/j.1432-1033.2002.03260.x

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SN - 0014-2956

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JO - European Journal of Biochemistry

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IS - 22

ER -

DNA and RNA damage by Cu(II)-amikacin complex

Abstract

Keywords

ASJC Scopus subject areas

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