DNA and RNA damage by Cu(II)-amikacin complex

Malgorzata Jezowska-Bojczuk, Wojciech Szczepanik, Wojciech Lesniak, Jerzy Ciesiolka, Jan Wrzesinski, Wojciech Bal

Research output: Contribution to journalArticlepeer-review

Abstract

The oxidation-promoting reactivity of copper(II) complex of aminoglycosidic antibiotic amikacin [Cu(II)-Ami] in the presence of hydrogen peroxide, was studied at pH 7.4, using 2′-deoxyguanosine (dG), pBR322 plasmid DNA and yeast tRNAPhe as target molecules. The mixtures of complex with H2O2 were found to be efficient oxidants, converting dG to its 8-oxo derivative, generating strand breaks in plasmid DNA and multiple cleavages in tRNAPhe. The complex underwent autooxidation as well, with amikacin hydroperoxides as likely major products. This reactivity pattern was found to be due to a combination of metal-bound and free hydroxyl radicals.

Original languageEnglish (US)
Pages (from-to)5547-5556
Number of pages10
JournalEuropean Journal of Biochemistry
Volume269
Issue number22
DOIs
StatePublished - 2002
Externally publishedYes

Keywords

  • 2′-deoxyguanosine oxidation
  • Amikacin
  • Copper(II) complexes
  • Plasmid DNA damage
  • tRNA cleavage

ASJC Scopus subject areas

  • Biochemistry

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