DNA adducts in urothelial cells: Relationship with biomarkers of exposure to arylamines and polycyclic aromatic hydrocarbons from tobacco smoke

Paolo Vineis, Glenn Talaska, Christian Malaveille, Helmut Bartsch, Tiziana Martone, Pornchai Sithisarankul, Paul Timothy Strickland

Research output: Contribution to journalArticle

Abstract

Markers of exposure to polycyclic aromatic hydrocarbons (urinary 1-hydroxypyrene-glucuronide) and aromatic amines (4-aminobiphenyl-hemoglobin adducts), as well as urinary mutagenicity, were measured in 47 healthy smokers and 50 nonsmokers. DNA adducts were determined by P32-postlabeling in the exfoliated bladder cells of 39 healthy subjects. Both 1-hydroxypyrene-glucuronide (I-OHPG) and 4-aminobiphenyl adducts (4-ABP-Hb) were associated with smoking habits, but only 4-ABP-Hb adducts were associated with consumption of black, air-cured tobacco. The levels of 2 DNA adducts (numbers 2 and 4) in urothelial cells were clearly associated with 4-ABP-Hb adducts, in all subjects and in smokers. Levels of one of these DNA adducts (number 2) were also associated with 1-hydroxypyrene-glucuronide in urines, but in smokers the association was not statistically significant. Overall, these observations constitute further evidence of a role of arylamines in tobacco-induced bladder cancer.

Original languageEnglish (US)
Pages (from-to)314-316
Number of pages3
JournalInternational Journal of Cancer
Volume65
Issue number3
DOIs
StatePublished - Jan 26 1996

Fingerprint

DNA Adducts
Polycyclic Aromatic Hydrocarbons
Smoke
Tobacco
Biomarkers
Urinary Bladder Neoplasms
Habits
Amines
Healthy Volunteers
Urinary Bladder
Smoking
Air
Urine
1-hydroxypyrene-glucuronide

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

DNA adducts in urothelial cells : Relationship with biomarkers of exposure to arylamines and polycyclic aromatic hydrocarbons from tobacco smoke. / Vineis, Paolo; Talaska, Glenn; Malaveille, Christian; Bartsch, Helmut; Martone, Tiziana; Sithisarankul, Pornchai; Strickland, Paul Timothy.

In: International Journal of Cancer, Vol. 65, No. 3, 26.01.1996, p. 314-316.

Research output: Contribution to journalArticle

Vineis, Paolo ; Talaska, Glenn ; Malaveille, Christian ; Bartsch, Helmut ; Martone, Tiziana ; Sithisarankul, Pornchai ; Strickland, Paul Timothy. / DNA adducts in urothelial cells : Relationship with biomarkers of exposure to arylamines and polycyclic aromatic hydrocarbons from tobacco smoke. In: International Journal of Cancer. 1996 ; Vol. 65, No. 3. pp. 314-316.
@article{fc5fa6a100004ef9a5f532a30d9f8600,
title = "DNA adducts in urothelial cells: Relationship with biomarkers of exposure to arylamines and polycyclic aromatic hydrocarbons from tobacco smoke",
abstract = "Markers of exposure to polycyclic aromatic hydrocarbons (urinary 1-hydroxypyrene-glucuronide) and aromatic amines (4-aminobiphenyl-hemoglobin adducts), as well as urinary mutagenicity, were measured in 47 healthy smokers and 50 nonsmokers. DNA adducts were determined by P32-postlabeling in the exfoliated bladder cells of 39 healthy subjects. Both 1-hydroxypyrene-glucuronide (I-OHPG) and 4-aminobiphenyl adducts (4-ABP-Hb) were associated with smoking habits, but only 4-ABP-Hb adducts were associated with consumption of black, air-cured tobacco. The levels of 2 DNA adducts (numbers 2 and 4) in urothelial cells were clearly associated with 4-ABP-Hb adducts, in all subjects and in smokers. Levels of one of these DNA adducts (number 2) were also associated with 1-hydroxypyrene-glucuronide in urines, but in smokers the association was not statistically significant. Overall, these observations constitute further evidence of a role of arylamines in tobacco-induced bladder cancer.",
author = "Paolo Vineis and Glenn Talaska and Christian Malaveille and Helmut Bartsch and Tiziana Martone and Pornchai Sithisarankul and Strickland, {Paul Timothy}",
year = "1996",
month = "1",
day = "26",
doi = "10.1002/(SICI)1097-0215(19960126)65:3<314::AID-IJC6>3.0.CO;2-2",
language = "English (US)",
volume = "65",
pages = "314--316",
journal = "International Journal of Cancer",
issn = "0020-7136",
publisher = "Wiley-Liss Inc.",
number = "3",

}

TY - JOUR

T1 - DNA adducts in urothelial cells

T2 - Relationship with biomarkers of exposure to arylamines and polycyclic aromatic hydrocarbons from tobacco smoke

AU - Vineis, Paolo

AU - Talaska, Glenn

AU - Malaveille, Christian

AU - Bartsch, Helmut

AU - Martone, Tiziana

AU - Sithisarankul, Pornchai

AU - Strickland, Paul Timothy

PY - 1996/1/26

Y1 - 1996/1/26

N2 - Markers of exposure to polycyclic aromatic hydrocarbons (urinary 1-hydroxypyrene-glucuronide) and aromatic amines (4-aminobiphenyl-hemoglobin adducts), as well as urinary mutagenicity, were measured in 47 healthy smokers and 50 nonsmokers. DNA adducts were determined by P32-postlabeling in the exfoliated bladder cells of 39 healthy subjects. Both 1-hydroxypyrene-glucuronide (I-OHPG) and 4-aminobiphenyl adducts (4-ABP-Hb) were associated with smoking habits, but only 4-ABP-Hb adducts were associated with consumption of black, air-cured tobacco. The levels of 2 DNA adducts (numbers 2 and 4) in urothelial cells were clearly associated with 4-ABP-Hb adducts, in all subjects and in smokers. Levels of one of these DNA adducts (number 2) were also associated with 1-hydroxypyrene-glucuronide in urines, but in smokers the association was not statistically significant. Overall, these observations constitute further evidence of a role of arylamines in tobacco-induced bladder cancer.

AB - Markers of exposure to polycyclic aromatic hydrocarbons (urinary 1-hydroxypyrene-glucuronide) and aromatic amines (4-aminobiphenyl-hemoglobin adducts), as well as urinary mutagenicity, were measured in 47 healthy smokers and 50 nonsmokers. DNA adducts were determined by P32-postlabeling in the exfoliated bladder cells of 39 healthy subjects. Both 1-hydroxypyrene-glucuronide (I-OHPG) and 4-aminobiphenyl adducts (4-ABP-Hb) were associated with smoking habits, but only 4-ABP-Hb adducts were associated with consumption of black, air-cured tobacco. The levels of 2 DNA adducts (numbers 2 and 4) in urothelial cells were clearly associated with 4-ABP-Hb adducts, in all subjects and in smokers. Levels of one of these DNA adducts (number 2) were also associated with 1-hydroxypyrene-glucuronide in urines, but in smokers the association was not statistically significant. Overall, these observations constitute further evidence of a role of arylamines in tobacco-induced bladder cancer.

UR - http://www.scopus.com/inward/record.url?scp=0030049526&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030049526&partnerID=8YFLogxK

U2 - 10.1002/(SICI)1097-0215(19960126)65:3<314::AID-IJC6>3.0.CO;2-2

DO - 10.1002/(SICI)1097-0215(19960126)65:3<314::AID-IJC6>3.0.CO;2-2

M3 - Article

C2 - 8575850

AN - SCOPUS:0030049526

VL - 65

SP - 314

EP - 316

JO - International Journal of Cancer

JF - International Journal of Cancer

SN - 0020-7136

IS - 3

ER -