Dm SAS is required for sialic acid biosynthesis in cultured Drosophila third instar larvae CNS neurons

Annelise E Von Bergen Granell, Karen B. Palter, Ihan Akan, Udayanath Aich, Kevin J Yarema, Michael J. Betenbaugh, William B. Thornhill, Esperanza Recio-Pinto

Research output: Contribution to journalArticle

Abstract

Sialylation is an important carbohydrate modification of glycoconjugates that has been shown to modulate many cellular/molecular interactions in vertebrates. In Drosophila melanogaster (Dm), using sequence homology, several enzymes of the sialylation pathway have been cloned and their function tested in expression systems. Here we investigated whether sialic acid incorporation in cultured Dm central nervous system (CNS) neurons required endogenously expressed Dm sialic acid synthase (DmSAS). We compared neurons derived from wild type Dm larvae with those containing a DmSAS mutation (148 bp deletion). The ability of these cells to produce Sia5NAz (sialic acid form) from Ac 4ManNAz (azide-derivatized N-acetylmannosamine) and incorporate it into their glycoconjugates was measured by tagging the azide group of Sia5NAz with fluorescent agents via Click-iT chemistry. We found that most of the wild type Dm CNS neurons incorporated Sia5NAz into their glycoconjugates. Sialic acid incorporation was higher at the soma than at the neurite and could also be detected at perinuclear regions and the plasma membrane. In contrast, neurons from the DmSAS mutant did not incorporate Sia5NAz unless DmSAS was reintroduced (rescue mutant). Most of the neurons expressed α2,6-sialyltransferase. These results confirm that the mutation was a null mutation and that no redundant sialic acid biosynthetic activity exists in Dm cells, i.e., there is only one DmSAS. They also provide the strongest proof to date that DmSAS is a key enzyme in the biosynthesis of sialic acids in Dm CNS neurons, and the observed subcellular distribution of the newly synthesized sialic acids offers insights into their biological function.

Original languageEnglish (US)
Pages (from-to)1287-1295
Number of pages9
JournalACS Chemical Biology
Volume6
Issue number11
DOIs
StatePublished - Nov 18 2011

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Biosynthesis
Neurology
N-Acetylneuraminic Acid
Drosophila melanogaster
Drosophila
Neurons
Larva
Central Nervous System
Glycoconjugates
Sialic Acids
Azides
Mutation
Sialyltransferases
Click Chemistry
Molecular interactions
Carisoprodol
Enzymes
Cell membranes
Fluorescent Dyes
Neurites

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine

Cite this

Granell, A. E. V. B., Palter, K. B., Akan, I., Aich, U., Yarema, K. J., Betenbaugh, M. J., ... Recio-Pinto, E. (2011). Dm SAS is required for sialic acid biosynthesis in cultured Drosophila third instar larvae CNS neurons. ACS Chemical Biology, 6(11), 1287-1295. https://doi.org/10.1021/cb200238k

Dm SAS is required for sialic acid biosynthesis in cultured Drosophila third instar larvae CNS neurons. / Granell, Annelise E Von Bergen; Palter, Karen B.; Akan, Ihan; Aich, Udayanath; Yarema, Kevin J; Betenbaugh, Michael J.; Thornhill, William B.; Recio-Pinto, Esperanza.

In: ACS Chemical Biology, Vol. 6, No. 11, 18.11.2011, p. 1287-1295.

Research output: Contribution to journalArticle

Granell, AEVB, Palter, KB, Akan, I, Aich, U, Yarema, KJ, Betenbaugh, MJ, Thornhill, WB & Recio-Pinto, E 2011, 'Dm SAS is required for sialic acid biosynthesis in cultured Drosophila third instar larvae CNS neurons', ACS Chemical Biology, vol. 6, no. 11, pp. 1287-1295. https://doi.org/10.1021/cb200238k
Granell, Annelise E Von Bergen ; Palter, Karen B. ; Akan, Ihan ; Aich, Udayanath ; Yarema, Kevin J ; Betenbaugh, Michael J. ; Thornhill, William B. ; Recio-Pinto, Esperanza. / Dm SAS is required for sialic acid biosynthesis in cultured Drosophila third instar larvae CNS neurons. In: ACS Chemical Biology. 2011 ; Vol. 6, No. 11. pp. 1287-1295.
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AU - Yarema, Kevin J

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