D,L-α-difluoromethylornithine, an irreversible inhibitor of ornithine decarboxylase, induces differentiation in MEL cells

Shailesh Kumar Choudhary, Dipali Sharma, Aparna Dixit

Research output: Contribution to journalArticlepeer-review

Abstract

In the present study, the effect of D,L-α-difluoromethylornithine (DFMO), an irreversible inhibitor of ornithine decarboxylase (ODC), on Friend's murine erythroleukemia (MEL) cell differentiation is investigated. DFMO was able to induce differentiation of MEL cells in culture as determined by haemoglobin (Hb) content and percentage of cells synthesizing Hb detected by benzidine staining. DFMO at a concentration of 2mM resulted in about 70% benzidine-positive cells on the fifth day. There was a time-dependent increase in the percentage of benzidine-positive cells starting from day three. However, only a 24 h presence of DFMO in the medium was required to induce differentiation suggesting that DFMO switches on a pathway during this period leading to terminal differentiation of MEL cells. DFMO induced differentiation of MEL cells was sensitive to dexamethasone and 5-bromo-2'-deoxyuridine. (C) 1999 Academic Press.

Original languageEnglish (US)
Pages (from-to)489-495
Number of pages7
JournalCell biology international
Volume23
Issue number7
DOIs
StatePublished - Jul 1999
Externally publishedYes

Keywords

  • D,L-α-difluoromethylornithine
  • Differentiation
  • Murine erythroleukemia cells

ASJC Scopus subject areas

  • Cell Biology

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