Diversity of anopheline species and their Plasmodium infection status in rural Bandarban, Bangladesh

Mohammad Shafiul Alam, Sumit Chakma, Wasif A. Khan, Gregory E. Glass, Abu Naser Mohon, Rubayet Elahi, Laura C. Norris, Milka Patracia Podder, Sabeena Ahmed, Rashidul Haque, David Allen Sack, David J Sullivan, Douglas Norris

Research output: Contribution to journalArticle

Abstract

Background: Historically, the Chittagong Hill Tracts (CHT) of Bangladesh was considered hyperendemic for malaria. To better understand the contemporary malaria epidemiology and to develop new and innovative control strategies, comprehensive epidemiologic studies are ongoing in two endemic unions of Bandarban district of CHT. Within these studies entomological surveillance has been undertaken to study the role of the existing anopheline species involved in the malaria transmission cycle throughout the year. Methods: CDC miniature light traps were deployed to collect anopheline mosquitoes from the sleeping room of the selected houses each month in a single union (Kuhalong). Molecular identification was carried out for available Anopheles species complexes. Circumsporozoite proteins (CSP) for Plasmodium falciparum, Plasmodium vivax-210 (Pv-210) and Plasmodium vivax-247(Pv-247) were detected by Enzyme-linked immunosorbent assay (ELISA) from the female anopheline mosquitoes. To confirm CSP-ELISA results, polymerase chain reaction (PCR) was also performed. Results: A total of 2,837 anopheline mosquitoes, of which 2,576 were female, belonging to 20 species were collected from July 2009 -June 2010. Anopheles jeyporiensis was the most abundant species (18.9%), followed by An. vagus (16.8%) and An. kochi (14.4%). ELISA was performed on 2,467 female mosquitoes of 19 species. 15 (0.6%) female anophelines belonging to eight species were found to be positive for Plasmodium infection by CSP-ELISA. Of those, 11 (0.4%) mosquitoes were positive for P. falciparum and four (0.2%) for Pv-210. No mosquito was found positive for Pv-247. An. maculatus (2.1%, 2/97) had the highest infection rate followed by An. umbrosus (1.7%, 2/115) and An. barbirostris (1.1%, 2/186). Other infected species were An. nigerrimus, An. nivipes, An. jeyporiensis, An. kochi, and An. vagus. Out of 11 P. falciparum CSP positive samples, seven turned out to be positive by PCR. None of the samples positive for Pv-210 was positive by PCR. In terms of abundance and incrimination, the results suggest that An. maculatus, An. jeyporiensis and An. nivipes play important roles in malaria transmission in Kuhalong. Conclusion: The findings of this study suggest that even in the presence of an insecticide impregnated bed-net intervention, a number of Anopheles species still play a role in the transmission of malaria. Further investigations are required to reveal the detailed biology and insecticide resistance patterns of the vector mosquito species in endemic areas in Bangladesh in order to assist with the planning and implementation of improved malaria control strategies.

Original languageEnglish (US)
Article number150
JournalParasites and Vectors
Volume5
Issue number1
DOIs
StatePublished - 2012

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Bangladesh
Plasmodium vivax
Malaria
Culicidae
Anopheles
Enzyme-Linked Immunosorbent Assay
Plasmodium falciparum
Polymerase Chain Reaction
Insecticide Resistance
Centers for Disease Control and Prevention (U.S.)
Insecticides
Epidemiologic Studies
Epidemiology
Proteins
Light
Infection

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

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Diversity of anopheline species and their Plasmodium infection status in rural Bandarban, Bangladesh. / Alam, Mohammad Shafiul; Chakma, Sumit; Khan, Wasif A.; Glass, Gregory E.; Mohon, Abu Naser; Elahi, Rubayet; Norris, Laura C.; Podder, Milka Patracia; Ahmed, Sabeena; Haque, Rashidul; Sack, David Allen; Sullivan, David J; Norris, Douglas.

In: Parasites and Vectors, Vol. 5, No. 1, 150, 2012.

Research output: Contribution to journalArticle

Alam, MS, Chakma, S, Khan, WA, Glass, GE, Mohon, AN, Elahi, R, Norris, LC, Podder, MP, Ahmed, S, Haque, R, Sack, DA, Sullivan, DJ & Norris, D 2012, 'Diversity of anopheline species and their Plasmodium infection status in rural Bandarban, Bangladesh', Parasites and Vectors, vol. 5, no. 1, 150. https://doi.org/10.1186/1756-3305-5-150
Alam, Mohammad Shafiul ; Chakma, Sumit ; Khan, Wasif A. ; Glass, Gregory E. ; Mohon, Abu Naser ; Elahi, Rubayet ; Norris, Laura C. ; Podder, Milka Patracia ; Ahmed, Sabeena ; Haque, Rashidul ; Sack, David Allen ; Sullivan, David J ; Norris, Douglas. / Diversity of anopheline species and their Plasmodium infection status in rural Bandarban, Bangladesh. In: Parasites and Vectors. 2012 ; Vol. 5, No. 1.
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title = "Diversity of anopheline species and their Plasmodium infection status in rural Bandarban, Bangladesh",
abstract = "Background: Historically, the Chittagong Hill Tracts (CHT) of Bangladesh was considered hyperendemic for malaria. To better understand the contemporary malaria epidemiology and to develop new and innovative control strategies, comprehensive epidemiologic studies are ongoing in two endemic unions of Bandarban district of CHT. Within these studies entomological surveillance has been undertaken to study the role of the existing anopheline species involved in the malaria transmission cycle throughout the year. Methods: CDC miniature light traps were deployed to collect anopheline mosquitoes from the sleeping room of the selected houses each month in a single union (Kuhalong). Molecular identification was carried out for available Anopheles species complexes. Circumsporozoite proteins (CSP) for Plasmodium falciparum, Plasmodium vivax-210 (Pv-210) and Plasmodium vivax-247(Pv-247) were detected by Enzyme-linked immunosorbent assay (ELISA) from the female anopheline mosquitoes. To confirm CSP-ELISA results, polymerase chain reaction (PCR) was also performed. Results: A total of 2,837 anopheline mosquitoes, of which 2,576 were female, belonging to 20 species were collected from July 2009 -June 2010. Anopheles jeyporiensis was the most abundant species (18.9{\%}), followed by An. vagus (16.8{\%}) and An. kochi (14.4{\%}). ELISA was performed on 2,467 female mosquitoes of 19 species. 15 (0.6{\%}) female anophelines belonging to eight species were found to be positive for Plasmodium infection by CSP-ELISA. Of those, 11 (0.4{\%}) mosquitoes were positive for P. falciparum and four (0.2{\%}) for Pv-210. No mosquito was found positive for Pv-247. An. maculatus (2.1{\%}, 2/97) had the highest infection rate followed by An. umbrosus (1.7{\%}, 2/115) and An. barbirostris (1.1{\%}, 2/186). Other infected species were An. nigerrimus, An. nivipes, An. jeyporiensis, An. kochi, and An. vagus. Out of 11 P. falciparum CSP positive samples, seven turned out to be positive by PCR. None of the samples positive for Pv-210 was positive by PCR. In terms of abundance and incrimination, the results suggest that An. maculatus, An. jeyporiensis and An. nivipes play important roles in malaria transmission in Kuhalong. Conclusion: The findings of this study suggest that even in the presence of an insecticide impregnated bed-net intervention, a number of Anopheles species still play a role in the transmission of malaria. Further investigations are required to reveal the detailed biology and insecticide resistance patterns of the vector mosquito species in endemic areas in Bangladesh in order to assist with the planning and implementation of improved malaria control strategies.",
author = "Alam, {Mohammad Shafiul} and Sumit Chakma and Khan, {Wasif A.} and Glass, {Gregory E.} and Mohon, {Abu Naser} and Rubayet Elahi and Norris, {Laura C.} and Podder, {Milka Patracia} and Sabeena Ahmed and Rashidul Haque and Sack, {David Allen} and Sullivan, {David J} and Douglas Norris",
year = "2012",
doi = "10.1186/1756-3305-5-150",
language = "English (US)",
volume = "5",
journal = "Parasites and Vectors",
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TY - JOUR

T1 - Diversity of anopheline species and their Plasmodium infection status in rural Bandarban, Bangladesh

AU - Alam, Mohammad Shafiul

AU - Chakma, Sumit

AU - Khan, Wasif A.

AU - Glass, Gregory E.

AU - Mohon, Abu Naser

AU - Elahi, Rubayet

AU - Norris, Laura C.

AU - Podder, Milka Patracia

AU - Ahmed, Sabeena

AU - Haque, Rashidul

AU - Sack, David Allen

AU - Sullivan, David J

AU - Norris, Douglas

PY - 2012

Y1 - 2012

N2 - Background: Historically, the Chittagong Hill Tracts (CHT) of Bangladesh was considered hyperendemic for malaria. To better understand the contemporary malaria epidemiology and to develop new and innovative control strategies, comprehensive epidemiologic studies are ongoing in two endemic unions of Bandarban district of CHT. Within these studies entomological surveillance has been undertaken to study the role of the existing anopheline species involved in the malaria transmission cycle throughout the year. Methods: CDC miniature light traps were deployed to collect anopheline mosquitoes from the sleeping room of the selected houses each month in a single union (Kuhalong). Molecular identification was carried out for available Anopheles species complexes. Circumsporozoite proteins (CSP) for Plasmodium falciparum, Plasmodium vivax-210 (Pv-210) and Plasmodium vivax-247(Pv-247) were detected by Enzyme-linked immunosorbent assay (ELISA) from the female anopheline mosquitoes. To confirm CSP-ELISA results, polymerase chain reaction (PCR) was also performed. Results: A total of 2,837 anopheline mosquitoes, of which 2,576 were female, belonging to 20 species were collected from July 2009 -June 2010. Anopheles jeyporiensis was the most abundant species (18.9%), followed by An. vagus (16.8%) and An. kochi (14.4%). ELISA was performed on 2,467 female mosquitoes of 19 species. 15 (0.6%) female anophelines belonging to eight species were found to be positive for Plasmodium infection by CSP-ELISA. Of those, 11 (0.4%) mosquitoes were positive for P. falciparum and four (0.2%) for Pv-210. No mosquito was found positive for Pv-247. An. maculatus (2.1%, 2/97) had the highest infection rate followed by An. umbrosus (1.7%, 2/115) and An. barbirostris (1.1%, 2/186). Other infected species were An. nigerrimus, An. nivipes, An. jeyporiensis, An. kochi, and An. vagus. Out of 11 P. falciparum CSP positive samples, seven turned out to be positive by PCR. None of the samples positive for Pv-210 was positive by PCR. In terms of abundance and incrimination, the results suggest that An. maculatus, An. jeyporiensis and An. nivipes play important roles in malaria transmission in Kuhalong. Conclusion: The findings of this study suggest that even in the presence of an insecticide impregnated bed-net intervention, a number of Anopheles species still play a role in the transmission of malaria. Further investigations are required to reveal the detailed biology and insecticide resistance patterns of the vector mosquito species in endemic areas in Bangladesh in order to assist with the planning and implementation of improved malaria control strategies.

AB - Background: Historically, the Chittagong Hill Tracts (CHT) of Bangladesh was considered hyperendemic for malaria. To better understand the contemporary malaria epidemiology and to develop new and innovative control strategies, comprehensive epidemiologic studies are ongoing in two endemic unions of Bandarban district of CHT. Within these studies entomological surveillance has been undertaken to study the role of the existing anopheline species involved in the malaria transmission cycle throughout the year. Methods: CDC miniature light traps were deployed to collect anopheline mosquitoes from the sleeping room of the selected houses each month in a single union (Kuhalong). Molecular identification was carried out for available Anopheles species complexes. Circumsporozoite proteins (CSP) for Plasmodium falciparum, Plasmodium vivax-210 (Pv-210) and Plasmodium vivax-247(Pv-247) were detected by Enzyme-linked immunosorbent assay (ELISA) from the female anopheline mosquitoes. To confirm CSP-ELISA results, polymerase chain reaction (PCR) was also performed. Results: A total of 2,837 anopheline mosquitoes, of which 2,576 were female, belonging to 20 species were collected from July 2009 -June 2010. Anopheles jeyporiensis was the most abundant species (18.9%), followed by An. vagus (16.8%) and An. kochi (14.4%). ELISA was performed on 2,467 female mosquitoes of 19 species. 15 (0.6%) female anophelines belonging to eight species were found to be positive for Plasmodium infection by CSP-ELISA. Of those, 11 (0.4%) mosquitoes were positive for P. falciparum and four (0.2%) for Pv-210. No mosquito was found positive for Pv-247. An. maculatus (2.1%, 2/97) had the highest infection rate followed by An. umbrosus (1.7%, 2/115) and An. barbirostris (1.1%, 2/186). Other infected species were An. nigerrimus, An. nivipes, An. jeyporiensis, An. kochi, and An. vagus. Out of 11 P. falciparum CSP positive samples, seven turned out to be positive by PCR. None of the samples positive for Pv-210 was positive by PCR. In terms of abundance and incrimination, the results suggest that An. maculatus, An. jeyporiensis and An. nivipes play important roles in malaria transmission in Kuhalong. Conclusion: The findings of this study suggest that even in the presence of an insecticide impregnated bed-net intervention, a number of Anopheles species still play a role in the transmission of malaria. Further investigations are required to reveal the detailed biology and insecticide resistance patterns of the vector mosquito species in endemic areas in Bangladesh in order to assist with the planning and implementation of improved malaria control strategies.

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