Diversification of the gut symbiont lactobacillus reuteri as a result of host-driven evolution

Phaik Lyn Oh, Andrew K. Benson, Daniel A. Peterson, Prabhu B. Patil, Etsuko N. Moriyama, Stefan Roos, Jens Walter

Research output: Contribution to journalArticle

Abstract

The vertebrate digestive tract, including that of humans, is the habitat to trillions of bacteria that are of significant importance to host biology and health. Although these communities are often postulated to have coevolved with their hosts, evidence is lacking, yet critical for our understanding of microbial symbiosis in vertebrates. To gain insight into the evolution of a gut symbiont, we have characterized the population genetic structure and phylogeny of Lactobacillus reuteri strains isolated from six different host species (human, mouse, rat, pig, chicken and turkey) using Amplified-Fragment Length Polymorphism (AFLP) and Multi-Locus Sequence Analysis (MLSA). The results revealed considerable genetic heterogeneity within the L. reuteri population and distinct monophyletic clades reflecting host origin but not provenance. The evolutionary patterns detected indicate a long-term association of L. reuteri lineages with particular vertebrate species and host-driven diversification. Results from a competition experiment in a gnotobiotic mouse model revealed that rodent isolates showed elevated ecological performance, indicating that evolution of L. reuteri lineages was adaptive. These findings provide evidence that some vertebrate gut microbes are not promiscuous, but have diversified into host-adapted lineages by a long-term evolutionary process, allowing the development of a highly specialized symbiosis.

Original languageEnglish (US)
Pages (from-to)377-387
Number of pages11
JournalISME Journal
Volume4
Issue number3
DOIs
StatePublished - Mar 2010
Externally publishedYes

Fingerprint

Lactobacillus reuteri
symbiont
symbionts
Vertebrates
digestive system
Symbiosis
vertebrate
vertebrates
Germ-Free Life
symbiosis
Genetic Heterogeneity
Genetic Structures
Population Genetics
Phylogeny
Ecosystem
Sequence Analysis
Gastrointestinal Tract
microsymbionts
Chickens
Rodentia

Keywords

  • Evolution
  • Lactobacillus reuteri
  • Microbiome
  • Population structure

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Microbiology

Cite this

Oh, P. L., Benson, A. K., Peterson, D. A., Patil, P. B., Moriyama, E. N., Roos, S., & Walter, J. (2010). Diversification of the gut symbiont lactobacillus reuteri as a result of host-driven evolution. ISME Journal, 4(3), 377-387. https://doi.org/10.1038/ismej.2009.123

Diversification of the gut symbiont lactobacillus reuteri as a result of host-driven evolution. / Oh, Phaik Lyn; Benson, Andrew K.; Peterson, Daniel A.; Patil, Prabhu B.; Moriyama, Etsuko N.; Roos, Stefan; Walter, Jens.

In: ISME Journal, Vol. 4, No. 3, 03.2010, p. 377-387.

Research output: Contribution to journalArticle

Oh, PL, Benson, AK, Peterson, DA, Patil, PB, Moriyama, EN, Roos, S & Walter, J 2010, 'Diversification of the gut symbiont lactobacillus reuteri as a result of host-driven evolution', ISME Journal, vol. 4, no. 3, pp. 377-387. https://doi.org/10.1038/ismej.2009.123
Oh, Phaik Lyn ; Benson, Andrew K. ; Peterson, Daniel A. ; Patil, Prabhu B. ; Moriyama, Etsuko N. ; Roos, Stefan ; Walter, Jens. / Diversification of the gut symbiont lactobacillus reuteri as a result of host-driven evolution. In: ISME Journal. 2010 ; Vol. 4, No. 3. pp. 377-387.
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