Diurnal variation in total and undercarboxylated osteocalcin: Influence of increased dietary phylloquinone

Lori J Sokoll, S. L. Booth, K. W. Davidson, G. E. Dallal, J. A. Sadowski

Research output: Contribution to journalArticle

Abstract

A diurnal variation exists in blood levels of the vitamin K-dependent bone protein osteocalcin. However, it is not known whether the carboxylated and undercarboxylated constituents of osteocalcin also vary. Therefore, osteocalcin and undercarboxylated osteocalcin were measured in specimens collected every 4 hours over a 24-hour period in nine healthy subjects (five males, four females) ages 20-33 years who were consuming a mixed diet containing 100 μg of phylloquinone. Osteocalcin and undercarboxylated osteocalcin were measured by radioimmunoassay (RIA) before and after treatment with barium sulfate. Although the percent undercarboxylated osteocalcin did not change, a diurnal variation was observed in total osteocalcin, carboxylated osteocalcin, and undercarboxylated osteocalcin, with peak concentrations at 4 a.m. and the lowest concentrations between 12 p.m. and 4 p.m. The difference between the total osteocalcin peak and trough concentrations averaged 28 ± 7 (SEM)%. There were no gender differences in these rhythms. The effect of dietary phylloquinone as a modulator of these rhythms was evaluated in a randomized study by increasing phylloquinone intake to 420 μg/day with fortified corn oil, split between the lunch and dinner meals. Total and carboxylated osteocalcin fluctuations and concentrations were not affected by the dietary treatment. The diurnal variation in undercarboxylated osteocalcin was abolished with supplementation and concentrations at 8 a.m. (14 hours following supplementation) (2.3 ± 0.2 ng/ml) were significantly lower than the unsupplemented levels (2.7 ± 0.2 ng/mL, P = 0.006). The percentage of undercarboxylated osteocalcin was similarly decreased after supplementation (19.7 ± 1.3%) in relation to the mixed diet cycle (24.2 ± 1.6%, P = 0.006) at 8 a.m. on the second day. Dietary supplementation induced a fluctuation in percentage undercarboxylated osteocalcin with a decline in levels starting at approximately 12 a.m. Therefore, additional dietary phylloquinone does not appear to modulate the total osteocalcin diurnal rhythm, but can influence its undercarboxylated component.

Original languageEnglish (US)
Pages (from-to)447-452
Number of pages6
JournalCalcified Tissue International
Volume62
Issue number5
DOIs
StatePublished - 1998

Fingerprint

Vitamin K 1
Osteocalcin
Meals
Barium Sulfate
Diet
Lunch
Corn Oil

Keywords

  • Diurnal variation
  • Osteocalcin
  • Phylloquinone
  • Undercarboxylated osteocalcin

ASJC Scopus subject areas

  • Endocrinology

Cite this

Diurnal variation in total and undercarboxylated osteocalcin : Influence of increased dietary phylloquinone. / Sokoll, Lori J; Booth, S. L.; Davidson, K. W.; Dallal, G. E.; Sadowski, J. A.

In: Calcified Tissue International, Vol. 62, No. 5, 1998, p. 447-452.

Research output: Contribution to journalArticle

Sokoll, Lori J ; Booth, S. L. ; Davidson, K. W. ; Dallal, G. E. ; Sadowski, J. A. / Diurnal variation in total and undercarboxylated osteocalcin : Influence of increased dietary phylloquinone. In: Calcified Tissue International. 1998 ; Vol. 62, No. 5. pp. 447-452.
@article{bfaf1147c83f47ec9913fe0dd9771a29,
title = "Diurnal variation in total and undercarboxylated osteocalcin: Influence of increased dietary phylloquinone",
abstract = "A diurnal variation exists in blood levels of the vitamin K-dependent bone protein osteocalcin. However, it is not known whether the carboxylated and undercarboxylated constituents of osteocalcin also vary. Therefore, osteocalcin and undercarboxylated osteocalcin were measured in specimens collected every 4 hours over a 24-hour period in nine healthy subjects (five males, four females) ages 20-33 years who were consuming a mixed diet containing 100 μg of phylloquinone. Osteocalcin and undercarboxylated osteocalcin were measured by radioimmunoassay (RIA) before and after treatment with barium sulfate. Although the percent undercarboxylated osteocalcin did not change, a diurnal variation was observed in total osteocalcin, carboxylated osteocalcin, and undercarboxylated osteocalcin, with peak concentrations at 4 a.m. and the lowest concentrations between 12 p.m. and 4 p.m. The difference between the total osteocalcin peak and trough concentrations averaged 28 ± 7 (SEM){\%}. There were no gender differences in these rhythms. The effect of dietary phylloquinone as a modulator of these rhythms was evaluated in a randomized study by increasing phylloquinone intake to 420 μg/day with fortified corn oil, split between the lunch and dinner meals. Total and carboxylated osteocalcin fluctuations and concentrations were not affected by the dietary treatment. The diurnal variation in undercarboxylated osteocalcin was abolished with supplementation and concentrations at 8 a.m. (14 hours following supplementation) (2.3 ± 0.2 ng/ml) were significantly lower than the unsupplemented levels (2.7 ± 0.2 ng/mL, P = 0.006). The percentage of undercarboxylated osteocalcin was similarly decreased after supplementation (19.7 ± 1.3{\%}) in relation to the mixed diet cycle (24.2 ± 1.6{\%}, P = 0.006) at 8 a.m. on the second day. Dietary supplementation induced a fluctuation in percentage undercarboxylated osteocalcin with a decline in levels starting at approximately 12 a.m. Therefore, additional dietary phylloquinone does not appear to modulate the total osteocalcin diurnal rhythm, but can influence its undercarboxylated component.",
keywords = "Diurnal variation, Osteocalcin, Phylloquinone, Undercarboxylated osteocalcin",
author = "Sokoll, {Lori J} and Booth, {S. L.} and Davidson, {K. W.} and Dallal, {G. E.} and Sadowski, {J. A.}",
year = "1998",
doi = "10.1007/s002239900458",
language = "English (US)",
volume = "62",
pages = "447--452",
journal = "Calcified Tissue International",
issn = "0171-967X",
publisher = "Springer New York",
number = "5",

}

TY - JOUR

T1 - Diurnal variation in total and undercarboxylated osteocalcin

T2 - Influence of increased dietary phylloquinone

AU - Sokoll, Lori J

AU - Booth, S. L.

AU - Davidson, K. W.

AU - Dallal, G. E.

AU - Sadowski, J. A.

PY - 1998

Y1 - 1998

N2 - A diurnal variation exists in blood levels of the vitamin K-dependent bone protein osteocalcin. However, it is not known whether the carboxylated and undercarboxylated constituents of osteocalcin also vary. Therefore, osteocalcin and undercarboxylated osteocalcin were measured in specimens collected every 4 hours over a 24-hour period in nine healthy subjects (five males, four females) ages 20-33 years who were consuming a mixed diet containing 100 μg of phylloquinone. Osteocalcin and undercarboxylated osteocalcin were measured by radioimmunoassay (RIA) before and after treatment with barium sulfate. Although the percent undercarboxylated osteocalcin did not change, a diurnal variation was observed in total osteocalcin, carboxylated osteocalcin, and undercarboxylated osteocalcin, with peak concentrations at 4 a.m. and the lowest concentrations between 12 p.m. and 4 p.m. The difference between the total osteocalcin peak and trough concentrations averaged 28 ± 7 (SEM)%. There were no gender differences in these rhythms. The effect of dietary phylloquinone as a modulator of these rhythms was evaluated in a randomized study by increasing phylloquinone intake to 420 μg/day with fortified corn oil, split between the lunch and dinner meals. Total and carboxylated osteocalcin fluctuations and concentrations were not affected by the dietary treatment. The diurnal variation in undercarboxylated osteocalcin was abolished with supplementation and concentrations at 8 a.m. (14 hours following supplementation) (2.3 ± 0.2 ng/ml) were significantly lower than the unsupplemented levels (2.7 ± 0.2 ng/mL, P = 0.006). The percentage of undercarboxylated osteocalcin was similarly decreased after supplementation (19.7 ± 1.3%) in relation to the mixed diet cycle (24.2 ± 1.6%, P = 0.006) at 8 a.m. on the second day. Dietary supplementation induced a fluctuation in percentage undercarboxylated osteocalcin with a decline in levels starting at approximately 12 a.m. Therefore, additional dietary phylloquinone does not appear to modulate the total osteocalcin diurnal rhythm, but can influence its undercarboxylated component.

AB - A diurnal variation exists in blood levels of the vitamin K-dependent bone protein osteocalcin. However, it is not known whether the carboxylated and undercarboxylated constituents of osteocalcin also vary. Therefore, osteocalcin and undercarboxylated osteocalcin were measured in specimens collected every 4 hours over a 24-hour period in nine healthy subjects (five males, four females) ages 20-33 years who were consuming a mixed diet containing 100 μg of phylloquinone. Osteocalcin and undercarboxylated osteocalcin were measured by radioimmunoassay (RIA) before and after treatment with barium sulfate. Although the percent undercarboxylated osteocalcin did not change, a diurnal variation was observed in total osteocalcin, carboxylated osteocalcin, and undercarboxylated osteocalcin, with peak concentrations at 4 a.m. and the lowest concentrations between 12 p.m. and 4 p.m. The difference between the total osteocalcin peak and trough concentrations averaged 28 ± 7 (SEM)%. There were no gender differences in these rhythms. The effect of dietary phylloquinone as a modulator of these rhythms was evaluated in a randomized study by increasing phylloquinone intake to 420 μg/day with fortified corn oil, split between the lunch and dinner meals. Total and carboxylated osteocalcin fluctuations and concentrations were not affected by the dietary treatment. The diurnal variation in undercarboxylated osteocalcin was abolished with supplementation and concentrations at 8 a.m. (14 hours following supplementation) (2.3 ± 0.2 ng/ml) were significantly lower than the unsupplemented levels (2.7 ± 0.2 ng/mL, P = 0.006). The percentage of undercarboxylated osteocalcin was similarly decreased after supplementation (19.7 ± 1.3%) in relation to the mixed diet cycle (24.2 ± 1.6%, P = 0.006) at 8 a.m. on the second day. Dietary supplementation induced a fluctuation in percentage undercarboxylated osteocalcin with a decline in levels starting at approximately 12 a.m. Therefore, additional dietary phylloquinone does not appear to modulate the total osteocalcin diurnal rhythm, but can influence its undercarboxylated component.

KW - Diurnal variation

KW - Osteocalcin

KW - Phylloquinone

KW - Undercarboxylated osteocalcin

UR - http://www.scopus.com/inward/record.url?scp=0031917773&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031917773&partnerID=8YFLogxK

U2 - 10.1007/s002239900458

DO - 10.1007/s002239900458

M3 - Article

C2 - 9541523

AN - SCOPUS:0031917773

VL - 62

SP - 447

EP - 452

JO - Calcified Tissue International

JF - Calcified Tissue International

SN - 0171-967X

IS - 5

ER -