Diurnal rhythmicity in intestinal SGLT-1 function, vmax, and mRNA expression topography

Ali Tavakkolizadeh, Urs V. Berger, K. Robert Shen, Lynne L. Levitsky, Michael J. Zinner, Matthias A. Hediger, Stanley W. Ashley, Edward E. Whang, David B. Rhoads

Research output: Contribution to journalArticle

Abstract

Mechanisms underlying the circadian rhythmicity in intestinal sugar absorption remain unclear. To test whether this rhythmicity is caused by changes in Na+-glucose cotransporter 1 (SGLT-1) function, we measured phloridzin-inhibitable sugar fluxes as an index of SGLT-1 activity. Jejunum obtained from rats killed at 6-h intervals during a 12-h light-dark cycle (CT0 is circadian time 0 h, time of light onset) were mounted in Ussing chambers, and 3-O-methylglucose (3-OMG) fluxes were calculated before and after addition of phloridzin. 3-OMG-induced change in short-circuit current and absorptive flux were significantly greater at CT9 than at CT3. This increase was phloridzin inhibitable. Kinetic studies indicated a significant increase in SGLT-1 maximal velocity (Vmax) at CT9. Food intake between CT3 and CT9 was <10% of the daily total, indicating that the increased SGLT-1 activity was anticipatory. Diurnicity of SGLT-1 mRNA was confirmed by Northern blotting. Expression topography analyzed by in situ hybridization revealed more intense labeling along the entire villus axis at CT9 and CT15 compared with CT3 and CT21. We conclude that diurnicity in intestinal sugar absorption is caused by periodicity in SGLT-1 Vmax.

Original languageEnglish (US)
Pages (from-to)G209-G215
JournalAmerican Journal of Physiology - Gastrointestinal and Liver Physiology
Volume280
Issue number2 43-2
StatePublished - Feb 1 2001

Keywords

  • Enzyme kinetics
  • In situ hybridization
  • Intestinal sugar absorption
  • Sodium-glucose cotransporter
  • Ussing chamber

ASJC Scopus subject areas

  • Physiology
  • Hepatology
  • Gastroenterology
  • Physiology (medical)

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    Tavakkolizadeh, A., Berger, U. V., Shen, K. R., Levitsky, L. L., Zinner, M. J., Hediger, M. A., Ashley, S. W., Whang, E. E., & Rhoads, D. B. (2001). Diurnal rhythmicity in intestinal SGLT-1 function, vmax, and mRNA expression topography. American Journal of Physiology - Gastrointestinal and Liver Physiology, 280(2 43-2), G209-G215.