Disulfiram reactivates latent HIV-1 in a Bcl-2-transduced primary CD4+ T cell model without inducing global T cell activation

Sifei Xing, Cynthia K. Bullen, Neeta S. Shroff, Liang Shan, Hung Chih Yang, Jordyn L. Manucci, Shridhar Bhat, Hao Zhang, Joseph Bernard Margolick, Thomas C Quinn, David M. Margolis, Janet M Siliciano, Robert F Siliciano

Research output: Contribution to journalArticle

Abstract

Highly active antiretroviral therapy (HAART) can reduce plasma HIV-1 levels to below the detection limit. However, due to the latent reservoir in resting CD4+ cells, HAART is not curative. Elimination of this reservoir is critical to curing HIV-1 infection. Agents that reactivate latent HIV-1 through nonspecific T cell activation are toxic. Here we demonstrate in a primary CD4+ T cell model that the FDA-approved drug disulfiram reactivates latent HIV-1 without global T cell activation. The extent to which disulfiram reactivates latent HIV-1 in patient cells is unclear, but the drug alone or in combination may be useful in future eradication strategies.

Original languageEnglish (US)
Pages (from-to)6060-6064
Number of pages5
JournalJournal of Virology
Volume85
Issue number12
DOIs
StatePublished - Jun 2011

Fingerprint

disulfiram
Disulfiram
Human immunodeficiency virus 1
HIV-1
T-lymphocytes
T-Lymphocytes
Highly Active Antiretroviral Therapy
drugs
therapeutics
Poisons
Pharmaceutical Preparations
HIV Infections
Limit of Detection
detection limit
cells
infection

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Disulfiram reactivates latent HIV-1 in a Bcl-2-transduced primary CD4+ T cell model without inducing global T cell activation. / Xing, Sifei; Bullen, Cynthia K.; Shroff, Neeta S.; Shan, Liang; Yang, Hung Chih; Manucci, Jordyn L.; Bhat, Shridhar; Zhang, Hao; Margolick, Joseph Bernard; Quinn, Thomas C; Margolis, David M.; Siliciano, Janet M; Siliciano, Robert F.

In: Journal of Virology, Vol. 85, No. 12, 06.2011, p. 6060-6064.

Research output: Contribution to journalArticle

@article{f7d71a30d45746d8a46b406cb7a74f64,
title = "Disulfiram reactivates latent HIV-1 in a Bcl-2-transduced primary CD4+ T cell model without inducing global T cell activation",
abstract = "Highly active antiretroviral therapy (HAART) can reduce plasma HIV-1 levels to below the detection limit. However, due to the latent reservoir in resting CD4+ cells, HAART is not curative. Elimination of this reservoir is critical to curing HIV-1 infection. Agents that reactivate latent HIV-1 through nonspecific T cell activation are toxic. Here we demonstrate in a primary CD4+ T cell model that the FDA-approved drug disulfiram reactivates latent HIV-1 without global T cell activation. The extent to which disulfiram reactivates latent HIV-1 in patient cells is unclear, but the drug alone or in combination may be useful in future eradication strategies.",
author = "Sifei Xing and Bullen, {Cynthia K.} and Shroff, {Neeta S.} and Liang Shan and Yang, {Hung Chih} and Manucci, {Jordyn L.} and Shridhar Bhat and Hao Zhang and Margolick, {Joseph Bernard} and Quinn, {Thomas C} and Margolis, {David M.} and Siliciano, {Janet M} and Siliciano, {Robert F}",
year = "2011",
month = "6",
doi = "10.1128/JVI.02033-10",
language = "English (US)",
volume = "85",
pages = "6060--6064",
journal = "Journal of Virology",
issn = "0022-538X",
publisher = "American Society for Microbiology",
number = "12",

}

TY - JOUR

T1 - Disulfiram reactivates latent HIV-1 in a Bcl-2-transduced primary CD4+ T cell model without inducing global T cell activation

AU - Xing, Sifei

AU - Bullen, Cynthia K.

AU - Shroff, Neeta S.

AU - Shan, Liang

AU - Yang, Hung Chih

AU - Manucci, Jordyn L.

AU - Bhat, Shridhar

AU - Zhang, Hao

AU - Margolick, Joseph Bernard

AU - Quinn, Thomas C

AU - Margolis, David M.

AU - Siliciano, Janet M

AU - Siliciano, Robert F

PY - 2011/6

Y1 - 2011/6

N2 - Highly active antiretroviral therapy (HAART) can reduce plasma HIV-1 levels to below the detection limit. However, due to the latent reservoir in resting CD4+ cells, HAART is not curative. Elimination of this reservoir is critical to curing HIV-1 infection. Agents that reactivate latent HIV-1 through nonspecific T cell activation are toxic. Here we demonstrate in a primary CD4+ T cell model that the FDA-approved drug disulfiram reactivates latent HIV-1 without global T cell activation. The extent to which disulfiram reactivates latent HIV-1 in patient cells is unclear, but the drug alone or in combination may be useful in future eradication strategies.

AB - Highly active antiretroviral therapy (HAART) can reduce plasma HIV-1 levels to below the detection limit. However, due to the latent reservoir in resting CD4+ cells, HAART is not curative. Elimination of this reservoir is critical to curing HIV-1 infection. Agents that reactivate latent HIV-1 through nonspecific T cell activation are toxic. Here we demonstrate in a primary CD4+ T cell model that the FDA-approved drug disulfiram reactivates latent HIV-1 without global T cell activation. The extent to which disulfiram reactivates latent HIV-1 in patient cells is unclear, but the drug alone or in combination may be useful in future eradication strategies.

UR - http://www.scopus.com/inward/record.url?scp=79958136377&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79958136377&partnerID=8YFLogxK

U2 - 10.1128/JVI.02033-10

DO - 10.1128/JVI.02033-10

M3 - Article

C2 - 21471244

AN - SCOPUS:79958136377

VL - 85

SP - 6060

EP - 6064

JO - Journal of Virology

JF - Journal of Virology

SN - 0022-538X

IS - 12

ER -