Disulfiram is a DNA demethylating agent and inhibits prostate cancer cell growth

Jianqing Lin, Michael C. Haffner, Yonggang Zhang, Byron H. Lee, W. Nathaniel Brennen, Justin Britton, Sushant K. Kachhap, Joong Sup Shim, Jun O. Liu, William G. Nelson, Srinivasan Yegnasubramanian, Michael A. Carducci

Research output: Contribution to journalArticle

Abstract

Background The clinical success of the nucleoside analogs 5-aza-cytidine (5-azaC) and 5-aza-2′deoxycytidine (5-aza-dC) as DNA methyltransferase (DNMT) inhibitors has spurred interest in the development of non-nucleoside inhibitors with improved pharmacologic and safety profiles. Because DNMT catalysis features attack of cytosine bases by an enzyme thiol group, we tested whether disulfiram (DSF), a thiol-reactive compound with known clinical safety, demonstrated DNMT inhibitory activity. Methods Inhibition of DNMT1 activity by DSF was assessed using methyltransferase activity assays with recombinant DNMT1. Next, prostate cancer cell lines were exposed to DSF and assessed for: i) reduction of global 5-methyl cytosine (5meC) content using liquid chromatography/tandem mass spectrometry (LC-MS/MS); ii) gene-specific promoter demethylation by methylation-specific PCR (MSP); and iii) gene-reactivation by real-time RT-PCR. DSF was also tested for growth inhibition using prostate cancer cell lines propagated in vitro in cell culture and in vivo as xenografts in nude mice. Results Disulfiram showed a dose-dependent inhibition of DNMT1 activity on a hemimethylated DNA substrate. In prostate cancer cells in culture, DSF exposure led to reduction of global genomic 5meC content, increase in unmethylated APC and RARB gene promoters, and associated re-expression of these genes, but did not significantly alter prostate-specific antigen (PSA) expression. DSF significantly inhibited growth and clonogenic survival of prostate cancer cell lines in culture and showed a trend for reduced growth of prostate cancer xenografts. Conclusions Disulfiram is a non-nucleoside DNMT1 inhibitor that can reduce global 5meC content, reactivate epigenetically silenced genes, and significantly inhibit growth in prostate cancer cell lines.

Original languageEnglish (US)
Pages (from-to)333-343
Number of pages11
JournalProstate
Volume71
Issue number4
DOIs
StatePublished - Mar 1 2011

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Keywords

  • DNA methylation
  • DNA methyltransferase inhibitor
  • Disulfiram
  • Prostate cancer

ASJC Scopus subject areas

  • Oncology
  • Urology

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