Distribution of neurofilament antigens after axonal injury

Jeffrey Rosenfeld, Mary Ellen Pease, John W. Griffin, Ludwig A. Sternberger, Nancy H. Sternberger, Donald L. Price

Research output: Contribution to journalArticle

Abstract

Phosphorylated and nonphosphorylated epitopes of neurofdament (NF) proteins are distributed in different regions of individual neurons. Immunocyto-chemical methods, with monoclonal antibodies directed against phosphorylated and nonphosphorylated NF, demonstrated nonphosphorylated NF in perikarya and proximal axonal segments of neurons in dorsal root ganglia, while phosphorylated NF proteins were present in axons of these cells. The distribution of these epitopes of NF were examined at various times following injury of axons in the rat sciatic nerve. Between one and 2 I days after crush of the proximal nerve, phosphorylated NF were present in neuronal perikarya. We have compared patterns of perikaryal immuno-reactivity at one time point (three weeks) following a more distal crush or complete transection of the sciatic nerve. At this time period, following transection/ligation, phosphorylated NF immunoreactivity was not present in perikarya, but abnormal staining was observed after nerve crush. These altered distributions of phosphorylated epitopes of NF are of interest because several recent reports have indicated that similar, but not identical, abnormal staining patterns occur in human neurological diseases, including Alzheimer’s disease and Parkinson’s disease. In accord with previous studies, this investigation indicates that one response of neurons to injury, or to disease, is an abnormal distribution of phosphorylated epitopes of NF proteins.

Original languageEnglish (US)
Pages (from-to)269-282
Number of pages14
JournalJournal of Neuropathology and Experimental Neurology
Volume46
Issue number3
StatePublished - 1987

Fingerprint

Intermediate Filaments
Epitopes
Nerve Crush
Antigens
Wounds and Injuries
Sciatic Nerve
Neurons
Axons
Staining and Labeling
Proteins
Spinal Ganglia
Ligation
Parkinson Disease
Alzheimer Disease
Monoclonal Antibodies

Keywords

  • Axotomy
  • Immunocytochemistry
  • Neurofilament
  • Phosphorylation

ASJC Scopus subject areas

  • Clinical Neurology
  • Pathology and Forensic Medicine
  • Cellular and Molecular Neuroscience
  • Neurology
  • Neuroscience(all)

Cite this

Rosenfeld, J., Pease, M. E., Griffin, J. W., Sternberger, L. A., Sternberger, N. H., & Price, D. L. (1987). Distribution of neurofilament antigens after axonal injury. Journal of Neuropathology and Experimental Neurology, 46(3), 269-282.

Distribution of neurofilament antigens after axonal injury. / Rosenfeld, Jeffrey; Pease, Mary Ellen; Griffin, John W.; Sternberger, Ludwig A.; Sternberger, Nancy H.; Price, Donald L.

In: Journal of Neuropathology and Experimental Neurology, Vol. 46, No. 3, 1987, p. 269-282.

Research output: Contribution to journalArticle

Rosenfeld, J, Pease, ME, Griffin, JW, Sternberger, LA, Sternberger, NH & Price, DL 1987, 'Distribution of neurofilament antigens after axonal injury', Journal of Neuropathology and Experimental Neurology, vol. 46, no. 3, pp. 269-282.
Rosenfeld J, Pease ME, Griffin JW, Sternberger LA, Sternberger NH, Price DL. Distribution of neurofilament antigens after axonal injury. Journal of Neuropathology and Experimental Neurology. 1987;46(3):269-282.
Rosenfeld, Jeffrey ; Pease, Mary Ellen ; Griffin, John W. ; Sternberger, Ludwig A. ; Sternberger, Nancy H. ; Price, Donald L. / Distribution of neurofilament antigens after axonal injury. In: Journal of Neuropathology and Experimental Neurology. 1987 ; Vol. 46, No. 3. pp. 269-282.
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