Distribucija glavnih gangliozida mozga u njušnom putu žaba

Translated title of the contribution: Distribution of major brain gangliosides in olfactory tract of frogs

Barbara Viljetić, Ivan Večeslav Degmečić, Vinko Krajina, Tomislav Bogdanović, Ana Mojsović-Ćuić, Domagoj Dikić, Katarina Vajn, Ronald Lee Schnaar, Marija Heffer

Research output: Contribution to journalArticle

Abstract

Gangliosides are major cell-surface determinants in the central nervous system (CNS) of vertebrates, found both in neuronal and glial cell membranes. Together with cholesterol and glycosylphosphatidylinositol (GPI) - anchored proteins, gangliosides are involved in organization of plasma membrane micro domains. Based on biochemical studies, frog brain was previously described as having low quantities of gangliosides and their distribution pattern in specific brain regions was unknown. Using highly specific monoclonal antibodies generated against four major brain gangliosides (GM1, GD1a, GD1b and GT1b), we examined the distribution of these molecules in CNS of four different species of frogs (Rana esculenta, Rana temporaria, Bufo bufo and Bufo viridis). We also studied the distribution of myelin- associated glycoprotein (MAG), an inhibitor of axonal regeneration, which is a ligand for gangliosides GD1a and GT1b. Our results show that ganglioside GD1a is expressed in neurons of olfactory bulb in all studied animals. In the brain of Rana sp., GD1a is expressed in the entire olfactory pathway, from olfactory bulbs to amygdala, while in Bufo sp. GD1a is restricted to the main olfactory bulb. Furthermore, we found that most of myelinated pathways in frogs express MAG, but do not express GD1a, which could be one of the reasons for better axon regeneration of neural pathways after CNS injury in amphibians in comparison to mammals.

Original languageUndefined
Pages (from-to)121-126
Number of pages6
JournalCollegium Antropologicum
Volume35
Issue numberSUPPL. 1
Publication statusPublished - Jan 2011

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Keywords

  • Amphibians
  • Gangliosides
  • Membrane microdomains
  • Myelin-associated glycoprotein (MAG)

ASJC Scopus subject areas

  • Anthropology

Cite this

Viljetić, B., Degmečić, I. V., Krajina, V., Bogdanović, T., Mojsović-Ćuić, A., Dikić, D., ... Heffer, M. (2011). Distribucija glavnih gangliozida mozga u njušnom putu žaba. Collegium Antropologicum, 35(SUPPL. 1), 121-126.