Distribution of histamine H3 binding in forebrain of mouse and guinea pig

Paul Cumming, Christine Laliberté, Albert Gjedde

Research output: Contribution to journalArticlepeer-review

Abstract

To map the unknown distribution of histamine H3 binding sites, we compared autoradiographs of the binding of the H3 agonist Nα-[3H]methylhistamine ([3H]NAMH) in the forebrain of mouse and guinea pig. Saturation binding revealed the presence of a single site with apparent affinity of 0.75 nM in the mouse and 1.2 nM in the guinea pig. Binding constants were nearly identical in male and female mice. The rank order of [3H]NAMH binding in mouse brain was striatum > insular cortex > neocortex, as previously observed in the rat. In the guinea pig, the rank order was insular cortex > striatum ≈ neocortex. The Bmax of [3H]NAMH in insular cortex of both species was close to 60 fmol/mg. Thus, the guinea pig differs from the rodent in that the highest density of H3 binding was present in cortical rather than subcortical structures.

Original languageEnglish (US)
Pages (from-to)276-279
Number of pages4
JournalBrain research
Volume664
Issue number1-2
DOIs
StatePublished - Nov 21 1994

Keywords

  • Basal ganglia
  • Brain
  • Guinea pig
  • H receptor
  • Histamine
  • Mouse

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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