Distribution of carbohydrate epitopes among disjoint subsets of leech sensory afferent neurons

Karl Zipser, Michelle Erhardt, Jianxiong Song, Robert N Cole, Birgit Zipser

Research output: Contribution to journalArticle

Abstract

Carbohydrate recognition plays an important role in the development of normal projections of sensory afferent neurons in the leech CNS. Four different carbohydrate epitopes are expressed by sensory afferents on their 130 kDa surface proteins: all sensory afferents share a common carbohydrate epitope (CE0) that helps them to enter and project diffusely across the synaptic neuropil; a restricted expression of three other carbohydrate epitopes (CE1, CE2, and CE3) serves to distinguish three subsets of sensory afferents. We examined the subsets of sensory afferents defined by their subset carbohydrate epitopes in the leech lip, skin, gut, and CNS. We established that the CE1, CE2, and CE3 subset epitopes define disjoint subsets of neurons by double labeling sensory afferents with monoclonal antibodies for different pairs of subset epitopes. We found that CE2 and CE3 afferents populate the lip and skin, but not the gut, and that these two subsets of sensory afferents have convergent projection patterns in the CNS. We found that CE1 afferents populate the gut and skin, but not lips; furthermore, their CNS projections diverge from those of CE2 and CE3 afferents. Our data fit the hypothesis that these carbohydrate epitopes are related to sensory modality of afferent subsets.

Original languageEnglish (US)
Pages (from-to)4481-4493
Number of pages13
JournalJournal of Neuroscience
Volume14
Issue number7
StatePublished - Jul 1994
Externally publishedYes

Fingerprint

Leeches
Afferent Neurons
Sensory Receptor Cells
Epitopes
Carbohydrates
Lip
Skin
Neuropil
Membrane Proteins
Monoclonal Antibodies
Neurons

Keywords

  • carbohydrates
  • development
  • lectin
  • leech
  • process outgrowth
  • surface glycoprotein
  • synapse formation

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Distribution of carbohydrate epitopes among disjoint subsets of leech sensory afferent neurons. / Zipser, Karl; Erhardt, Michelle; Song, Jianxiong; Cole, Robert N; Zipser, Birgit.

In: Journal of Neuroscience, Vol. 14, No. 7, 07.1994, p. 4481-4493.

Research output: Contribution to journalArticle

Zipser, K, Erhardt, M, Song, J, Cole, RN & Zipser, B 1994, 'Distribution of carbohydrate epitopes among disjoint subsets of leech sensory afferent neurons', Journal of Neuroscience, vol. 14, no. 7, pp. 4481-4493.
Zipser, Karl ; Erhardt, Michelle ; Song, Jianxiong ; Cole, Robert N ; Zipser, Birgit. / Distribution of carbohydrate epitopes among disjoint subsets of leech sensory afferent neurons. In: Journal of Neuroscience. 1994 ; Vol. 14, No. 7. pp. 4481-4493.
@article{127ecd49fcf6421c9adb6564464a9d09,
title = "Distribution of carbohydrate epitopes among disjoint subsets of leech sensory afferent neurons",
abstract = "Carbohydrate recognition plays an important role in the development of normal projections of sensory afferent neurons in the leech CNS. Four different carbohydrate epitopes are expressed by sensory afferents on their 130 kDa surface proteins: all sensory afferents share a common carbohydrate epitope (CE0) that helps them to enter and project diffusely across the synaptic neuropil; a restricted expression of three other carbohydrate epitopes (CE1, CE2, and CE3) serves to distinguish three subsets of sensory afferents. We examined the subsets of sensory afferents defined by their subset carbohydrate epitopes in the leech lip, skin, gut, and CNS. We established that the CE1, CE2, and CE3 subset epitopes define disjoint subsets of neurons by double labeling sensory afferents with monoclonal antibodies for different pairs of subset epitopes. We found that CE2 and CE3 afferents populate the lip and skin, but not the gut, and that these two subsets of sensory afferents have convergent projection patterns in the CNS. We found that CE1 afferents populate the gut and skin, but not lips; furthermore, their CNS projections diverge from those of CE2 and CE3 afferents. Our data fit the hypothesis that these carbohydrate epitopes are related to sensory modality of afferent subsets.",
keywords = "carbohydrates, development, lectin, leech, process outgrowth, surface glycoprotein, synapse formation",
author = "Karl Zipser and Michelle Erhardt and Jianxiong Song and Cole, {Robert N} and Birgit Zipser",
year = "1994",
month = "7",
language = "English (US)",
volume = "14",
pages = "4481--4493",
journal = "Journal of Neuroscience",
issn = "0270-6474",
publisher = "Society for Neuroscience",
number = "7",

}

TY - JOUR

T1 - Distribution of carbohydrate epitopes among disjoint subsets of leech sensory afferent neurons

AU - Zipser, Karl

AU - Erhardt, Michelle

AU - Song, Jianxiong

AU - Cole, Robert N

AU - Zipser, Birgit

PY - 1994/7

Y1 - 1994/7

N2 - Carbohydrate recognition plays an important role in the development of normal projections of sensory afferent neurons in the leech CNS. Four different carbohydrate epitopes are expressed by sensory afferents on their 130 kDa surface proteins: all sensory afferents share a common carbohydrate epitope (CE0) that helps them to enter and project diffusely across the synaptic neuropil; a restricted expression of three other carbohydrate epitopes (CE1, CE2, and CE3) serves to distinguish three subsets of sensory afferents. We examined the subsets of sensory afferents defined by their subset carbohydrate epitopes in the leech lip, skin, gut, and CNS. We established that the CE1, CE2, and CE3 subset epitopes define disjoint subsets of neurons by double labeling sensory afferents with monoclonal antibodies for different pairs of subset epitopes. We found that CE2 and CE3 afferents populate the lip and skin, but not the gut, and that these two subsets of sensory afferents have convergent projection patterns in the CNS. We found that CE1 afferents populate the gut and skin, but not lips; furthermore, their CNS projections diverge from those of CE2 and CE3 afferents. Our data fit the hypothesis that these carbohydrate epitopes are related to sensory modality of afferent subsets.

AB - Carbohydrate recognition plays an important role in the development of normal projections of sensory afferent neurons in the leech CNS. Four different carbohydrate epitopes are expressed by sensory afferents on their 130 kDa surface proteins: all sensory afferents share a common carbohydrate epitope (CE0) that helps them to enter and project diffusely across the synaptic neuropil; a restricted expression of three other carbohydrate epitopes (CE1, CE2, and CE3) serves to distinguish three subsets of sensory afferents. We examined the subsets of sensory afferents defined by their subset carbohydrate epitopes in the leech lip, skin, gut, and CNS. We established that the CE1, CE2, and CE3 subset epitopes define disjoint subsets of neurons by double labeling sensory afferents with monoclonal antibodies for different pairs of subset epitopes. We found that CE2 and CE3 afferents populate the lip and skin, but not the gut, and that these two subsets of sensory afferents have convergent projection patterns in the CNS. We found that CE1 afferents populate the gut and skin, but not lips; furthermore, their CNS projections diverge from those of CE2 and CE3 afferents. Our data fit the hypothesis that these carbohydrate epitopes are related to sensory modality of afferent subsets.

KW - carbohydrates

KW - development

KW - lectin

KW - leech

KW - process outgrowth

KW - surface glycoprotein

KW - synapse formation

UR - http://www.scopus.com/inward/record.url?scp=0028289958&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028289958&partnerID=8YFLogxK

M3 - Article

C2 - 7517997

AN - SCOPUS:0028289958

VL - 14

SP - 4481

EP - 4493

JO - Journal of Neuroscience

JF - Journal of Neuroscience

SN - 0270-6474

IS - 7

ER -